Table 9.
Top 30 hypomethylated CpG sites (P < 6.50E–03) located within gene body regions in PNS cows compared with Control cows at 5 years of age
| BTAa | Mb | Differenceb | Gene |
|---|---|---|---|
| 18 | 46.4 | −18.75 | ATPase H+/K+ transporting subunit alpha (ATP4A) |
| 15 | 78.4 | −10.13 | SPI-1 proto-oncogene (SPI1) |
| 23 | 49.5 | −15.36 | Chromodomain Y like (CDYL) |
| 6 | 91.1 | −13.77 | Amphiregulin (AREG) |
| 22 | 2.9 | −17.73 | Zinc finger CW-type and PWWP domain containing 2 (ZCWPW2) |
| 4 | 98.8 | −19.07 | Leucine rich repeats and guanylate kinase domain containing (LRGUK) |
| 20 | 41.0 | −17.88 | Natriuretic peptide receptor 3 (NPR3) |
| 21 | 34.8 | −11.25 | Coiled-coil domain containing 33 (CCDC33) |
| 17 | 47.1 | −45.21 | Adhesion G protein-coupled receptor D1 (ADGRD1) |
| X | 100.1 | −10.72 | Moesin (MSN) |
| 11 | 10.1 | −17.24 | Ladybird homeobox 2 (LBX2) |
| 20 | 2.1 | −11.14 | Lymphocyte cytosolic protein 2 (LCP2) |
| 8 | 71.7 | −11.97 | NK2 homeobox 6 (NKX2-6) |
| 9 | 26.4 | −17.24 | TPD52 like 1 (TPD52 L1) |
| 10 | 32.9 | −10.81 | Meis homeobox 2 (MEIS2) |
| 26 | 11.0 | −12.85 | Cholesterol 25-hydroxylase (CH25H) |
| 16 | 69.6 | −23.49 | Phospholipase A2 group IVA (PLA2G4A) |
| 20 | 23.5 | −11.91 | Solute carrier family 38 member 9 (SLC38A9) |
| 8 | 53.3 | −15.60 | Forkhead box B2 (FOXB2) |
| 25 | 4.0 | −17.88 | Periplakin (PPL) |
| 25 | 0.5 | −17.94 | RAB40C, member RAS oncogene family (RAB40C) |
| 28 | 1.3 | −38.23 | Polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2) |
| 7 | 45.7 | −11.43 | One cut homeobox 3 (ONECUT3) |
| 23 | 49.2 | −41.33 | Phenylalanyl-TRNA synthetase 2, mitochondrial (FARS2) |
| 20 | 3.0 | −17.19 | RAN binding protein 17 (RANBP17) |
| 5 | 30.7 | −29.53 | Complement C1q like 4 (C1QL4) |
| X | 56.9 | −22.06 | Ring finger protein 128 (RNF128) |
| 28 | 7.0 | −11.04 | Solute carrier family 35 member F3 (SLC35F3) |
| 8 | 107.6 | −13.59 | Astrotactin 2 (ASTN2) |
| 22 | 2.9 | −14.56 | Zinc finger CW-type and PWWP domain containing 2 (ZCWPW2) |
aBTA: Bos taurus autosome. X indicates Bos taurus X chromosome. DNA methylation in bolded chromosomes was located within exons, otherwise within introns.
bMethylation difference was calculated by subtracting the mean methylation ratio in Control at 5 years of age from the mean methylation ratio in PNS at 5 years of age.