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. 2021 Apr 20;13(1):1912884. doi: 10.1080/19420862.2021.1912884

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Screening human PBMCs for HPIV3-specific B cells. HPIV3-specific B cells were labeled with APC-conjugated streptavidin tetramers of biotinylated HPIV3 prefusion (preF) protein followed by magnetic enrichment using microbeads against APC. Representative flow cytometry plot of enriched HPIV3-specific B cells after gating on live (fixable viability dye negative), CD3/CD14/CD16⁻, and CD19⁺ B cells. Cells in the red box of the enriched fraction are B cells that bind the preF but not postfusion (postF) conformation of the HPIV3 F protein. The percentage is of total cells shown in the flow plot. N = 6 donors