Antibodies |
|
|
Akt T308 AF647 C31E5E |
Cell Signaling |
48646 |
Akt S473 AF647 D9E |
Cell Signaling |
40755 |
Akt Total 9272 |
Cell Signaling |
9272S |
Akt S473 9271 |
Cell Signaling |
9271S |
Anti-BRDU APC BU20A |
eBioscience |
17-5071-42 |
Anti-BRDU APC 51-23619L |
BD |
552598 |
B220 PE-Cy7 RA3-6B2 |
eBioscience |
25-0452-82 |
B220 APC-Cy7 RA3-6B2 |
BioLegend |
103223 |
CCR6 APC 292L17 |
BioLegend |
129813 |
CCR6 BV421 140706 |
BD |
564736 |
CD11b APC-Cy7 M1/70 |
Tonbo |
25–0112 |
CD11c APC-Cy7 N418 |
Tonbo |
25–0114 |
CD19 PerCP-Cy5 6D5 |
BioLegend |
115533 |
CD25 PE PC61.5 |
Tonbo |
50–0251 |
CD4 PE-Cy7 RM4-5 |
Tonbo |
60–0042 |
CD4 PE-Cy7 RM4-5 |
BD |
552775 |
CD4 PE-Cy7 GK1.5 |
Tonbo |
60–0041 |
CD4 e450 GK1.5 |
eBioscience |
48-0041-82 |
CD4 PE GK1.5 |
BD |
557308 |
CD44 FITC IM7 |
eBioscience |
11-0441-82 |
CD45 APC-Cy7 30-F11 |
BioLegend |
103115 |
CD45.1 FITC A20 |
BD |
553775 |
CD45.2 BV570 104 |
BioLegend |
109833 |
CD62l APC MEL-14 |
eBioscience |
17-0621-81 |
CD8α PE 53–6.7 |
eBioscience |
12-0081-82 |
CD8α APC 53–6.7 |
eBioscience |
17-0081-82 |
CD8α APC-Cy7 53–6.7 |
Tonbo |
25–0081 |
Cell Trace Violet 405/450 |
ThermoFisher |
C34571 |
CXCR3 PE CXCR3–173 |
eBioscience |
12-1831-82 |
Fixable Dead Cell Dye Near IR 633/775 |
ThermoFisher |
L10119 |
Foxp3 FITC FJK-16S |
eBioscience |
11-5773-82 |
Foxp3 PE FJK-16S |
eBioscience |
12–5773-82 |
Gata3 AF488 L50–823 |
BD |
560077 |
Gata3 PE TWAJ |
eBioscience |
12-9966-42 |
GM-CSF PE MP1–27E9 |
eBioscience |
12-7331-82 |
IL-10 PE Jes5–16E3 |
eBioscience |
12-7101-41 |
IL-17a PE ebio17B7 |
eBioscience |
12-7177-81 |
IL-17a APC ebio17B7 |
eBioscience |
17-7177-81 |
IL-17a AF488 TC11-18H10 |
BD |
560220 |
IL-17a PerCP-Cy5 TC11-18H10 |
BD |
560666 |
IL-22 PE 1H8PWSR |
eBioscience |
12-7221-82 |
IL-4 FITC BVDG-2462 |
eBioscience |
11-7042-41 |
IL-4 APC 11B11 |
eBioscience |
17-7041-82 |
IFNγ PE XMG1.2 |
eBioscience |
12-7311-82 |
IFNγ PE-Cy7 XMG1.2 |
eBioscience |
25-7311-41 |
IFNγ APC XMG1.2 |
eBioscience |
17-7311-82 |
IFNγ PerCP-Cy5 XMG1.2 |
BD |
560660 |
Ki67 PE SolA15 |
eBioscience |
12-5698-82 |
NK1.1 APC PK136 |
BioLegend |
108710 |
NK1.1 APC-Cy7 PK136 |
BioLegend |
108724 |
P70 S6K Total 49D7 |
Cell Signaling |
2708S |
P70 S6K T389 1A5 |
Cell Signaling |
9206S |
P70 S6K T421/S424 9204 |
Cell Signaling |
9204S |
RORγt PE AFKJS-9 |
eBioscience |
12-6988-82 |
RORγt APC AFKJS-9 |
eBioscience |
17-6988-82 |
S6 S235/236 Pacific Blue D57.2.2E |
Cell Signaling |
8520S |
Streptavidin PE-Cy7 |
BD |
405206 |
Tbet PE eBio4B110 |
eBioscience |
12-5825-82 |
Tbet APC eBio4B110 |
eBioscience |
17-5825-82 |
Thy1.1 APC 53–2.1 |
BD |
561974 |
Thy1.1 PerCP-Cy5 HIS51 |
eBioscience |
45-0900-82 |
Chemicals, Peptides, and Recombinant Proteins |
|
|
IL-1β |
R&D |
401-ML-025 |
IL-12 |
R&D |
419-ML-050 |
IL-23 |
R&D |
1887-ML-010 |
IL-4 |
R&D |
404-ML-10 |
IL-6 |
R&D |
406-ML-005 |
Tgfβ |
R&D |
240-B-10 |
Igf |
Gropep |
CU020 |
Igf2 |
Gropep |
FU020 |
desIgf1 |
Gropep |
DU100 |
desIgf2 |
Gropep |
MU020 |
Golgiplug |
BD |
|
Phorbol 12-myristate 13-acetate |
Sigma-Aldrich |
P185–1MG |
Ionomycin |
EMD Bioscience |
4D7–952 |
Critical Commercial Assays |
|
|
CD4+ T Cell Isolation Kit |
Miltenyi |
130-104-454 |
LS Columns |
Miltenyi |
130-042-401 |
Dyna Beads |
Invitrogen |
11445D |
SsoAdvanced Universal SYBR Green Supermix |
Bio-Rad |
1725275 |
iScript cDNA Synthesis Kit |
Bio-Rad |
1708891 |
MOG35-55/CFA Emulsion PTX |
Hooke Labs |
EK-2160 |
Mitochondrial Stress Test |
Agilent |
105015–100 |
Glycolytic Rate Assay |
Agilent |
103344–100 |
Qiazol |
Qiagen |
217084 |
miRNeasy |
Qiagen |
79306 |
TruSeq Stranded mRNA Library Prep Kit |
Illumina |
RS-122-2103 |
Deposited Data |
|
|
RNA-Seq, CD4 T Cells |
GEO Database |
GSE114733 |
RNA-Seq, Innate Lymphooid Cells |
GEO Database |
GSE37448 |
Experimental Models: Cell Lines |
|
|
Mouse primary T lymphocytes |
Generated in house |
|
Experimental Models: Organisms/Strains |
|
|
Igf1rtm1Jcbr
|
Kloting et al., 2008 |
MGI:3818453 |
Igf1rtm2Arge/J |
Jackson Laboratory |
Stock No: 012251 |
Foxp3tm2Ayr
|
Fontenot et al., 2005 |
MGI:3574964 |
B6-LY5.2/Cr |
Charles River |
Strain Code 564 |
B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J |
Jackson Laboratory |
Stock #022071 |
10BiT Tg(Il10-Thy1a)1Weav |
Generated in house |
MGI:3767675 |
IFNγ.Thy1.1 (Ifngtm1(Thy1)Weav) |
Generated in house |
MGI:3831296 |
IL-17a.hCD2 |
Generated in house |
N/A |
RorcCre/+
|
Provided by Gerard Eberl |
MGI:3054098 |
Oligonucleotides |
|
|
B2m Forward GGTCTTTCTGGTGCTTGTCT |
Generated in house |
|
B2m Reverse TATGTTCGGCTTCCCATTCTC |
Generated in house |
|
Csf2 Forward GAAGATATTCGAGCAGGGTCTAC |
Generated in house |
|
Csf2 Reverse CTTGTGTTTCACAGTCCGTTTC |
Generated in house |
|
Foxp3 Forward CCCAGAGTTCTTCCACAACAT |
Generated in house |
|
Foxp3 Reverse TTGAGTGTCCTCTGCCTCT |
Generated in house |
|
Gata3 Forward TCTGGAGGAGGAACGCTAAT |
Generated in house |
|
Gata3 Reverse GGTCTGGATGCCTTCTTTCTT |
Generated in house |
|
Ifng Forward CTCTTCCTCATGGCTGTTTCT |
Generated in house |
|
Ifng Reverse TTCTTCCACATCTATGCCACTT |
Generated in house |
|
Igf1r Forward ACCATCGATTCGGTGACTTCTGCT |
Generated in house |
|
Igf1r Reverse AAGGACAAGGAGACCAAGGCATGA |
Generated in house |
|
Igf2r Forward AACATTTGTGTGCCCATCTGAGCG |
Generated in house |
|
Igf2r Reverse ATTGGGCCAAGGGACTAAGGTCAA |
Generated in house |
|
Igfbp4 Forward TCGGAAATCGAAGCCATCCAGGAA |
Generated in house |
|
Igfbp4 Reverse GGGTTGAAGCTGTTGTTGGGATGT |
Generated in house |
|
Il3 Forward AGGACCCTCTCTGAGGAATAAG |
Generated in house |
|
Il3 Reverse TGTAGGCAGGCAACAGTTAAG |
Generated in house |
|
Il10 Forward TTGAATTCCCTGGGTGAGAAG |
Generated in house |
|
Il10 Reverse TCCACTGCCTTGCTCTTATTT |
Generated in house |
|
Il17a Forward CAAACATGAGTCCAGGGAGAG |
Generated in house |
|
Il17a Reverse GCTGAGCTTTGAGGGATGAT |
Generated in house |
|
Il22 Forward CGACCAGAACATCCAGAAGAA |
Generated in house |
|
Il22 Reverse GAGACATAAACAGCAGGTCCA |
Generated in house |
|
Rorgt Ex2–4 Forward GAAGACCCACACCTCACAAA |
Generated in house |
|
Rorgt Ex2–4 Reverse CAGGAGTAGGCCACATTACAC |
Generated in house |
|
Rorgt Ex10–11 Forward TGCAAGACTCATCGACAAGG |
Generated in house |
|
Rorgt Ex10–11 Reverse TCCTTATAGAGTGGAGGGAAGG |
Generated in house |
|
Tbx21 Forward AGTTCAACCAGCACCAGAC |
Generated in house |
|
Tbx21 Reverse CACATCCACAAACATCCTGTAATG |
Generated in house |
|
Tgfb3 Forward CGCTACATAGGTGGCAAGAA |
Generated in house |
|
Tgfb3 Reverse CAAGTTGGACTCTCTCCTCAAC |
Generated in house |
|
Recombinant DNA |
|
|
Mouse IL-17a BAC |
CHORI |
RP24–305023 |
pL451 |
Liu et al. 2003 |
N/A |
pL253 |
Na et al. 2013 |
N/A |
pBSK |
Addgene |
67504 |
Human CD2 Y91D |
Wolff et al. 1990 |
N/A |
Software and Algorithms |
|
|
STAR (version 2.5.3) |
Dobin et al., 2013 |
|
SAMtools (version 0.1.18) |
Li et al., 2009 |
|
HTSeq (version 0.7.2) |
Anders et al., 2015 |
|
DESeq2 (version 1.18.1) |
Love et al., 2014 |
|
R (version 3.4.3) |
Htt[s://www.r-project.org |
|
ASHR |
Stephens et al., 2017 |
|
fgsea R package (version 1.4.0) |
https://github.com/ctlab/fgsea/ |
|
MSigDB |
Liberzon et al., 2011 |
|