KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Akt T308 AF647 C31E5E | Cell Signaling | 48646 |
| Akt S473 AF647 D9E | Cell Signaling | 40755 |
| Akt Total 9272 | Cell Signaling | 9272S |
| Akt S473 9271 | Cell Signaling | 9271S |
| Anti-BRDU APC BU20A | eBioscience | 17-5071-42 |
| Anti-BRDU APC 51-23619L | BD | 552598 |
| B220 PE-Cy7 RA3-6B2 | eBioscience | 25-0452-82 |
| B220 APC-Cy7 RA3-6B2 | BioLegend | 103223 |
| CCR6 APC 292L17 | BioLegend | 129813 |
| CCR6 BV421 140706 | BD | 564736 |
| CD11b APC-Cy7 M1/70 | Tonbo | 25–0112 |
| CD11c APC-Cy7 N418 | Tonbo | 25–0114 |
| CD19 PerCP-Cy5 6D5 | BioLegend | 115533 |
| CD25 PE PC61.5 | Tonbo | 50–0251 |
| CD4 PE-Cy7 RM4-5 | Tonbo | 60–0042 |
| CD4 PE-Cy7 RM4-5 | BD | 552775 |
| CD4 PE-Cy7 GK1.5 | Tonbo | 60–0041 |
| CD4 e450 GK1.5 | eBioscience | 48-0041-82 |
| CD4 PE GK1.5 | BD | 557308 |
| CD44 FITC IM7 | eBioscience | 11-0441-82 |
| CD45 APC-Cy7 30-F11 | BioLegend | 103115 |
| CD45.1 FITC A20 | BD | 553775 |
| CD45.2 BV570 104 | BioLegend | 109833 |
| CD62l APC MEL-14 | eBioscience | 17-0621-81 |
| CD8α PE 53–6.7 | eBioscience | 12-0081-82 |
| CD8α APC 53–6.7 | eBioscience | 17-0081-82 |
| CD8α APC-Cy7 53–6.7 | Tonbo | 25–0081 |
| Cell Trace Violet 405/450 | ThermoFisher | C34571 |
| CXCR3 PE CXCR3–173 | eBioscience | 12-1831-82 |
| Fixable Dead Cell Dye Near IR 633/775 | ThermoFisher | L10119 |
| Foxp3 FITC FJK-16S | eBioscience | 11-5773-82 |
| Foxp3 PE FJK-16S | eBioscience | 12–5773-82 |
| Gata3 AF488 L50–823 | BD | 560077 |
| Gata3 PE TWAJ | eBioscience | 12-9966-42 |
| GM-CSF PE MP1–27E9 | eBioscience | 12-7331-82 |
| IL-10 PE Jes5–16E3 | eBioscience | 12-7101-41 |
| IL-17a PE ebio17B7 | eBioscience | 12-7177-81 |
| IL-17a APC ebio17B7 | eBioscience | 17-7177-81 |
| IL-17a AF488 TC11-18H10 | BD | 560220 |
| IL-17a PerCP-Cy5 TC11-18H10 | BD | 560666 |
| IL-22 PE 1H8PWSR | eBioscience | 12-7221-82 |
| IL-4 FITC BVDG-2462 | eBioscience | 11-7042-41 |
| IL-4 APC 11B11 | eBioscience | 17-7041-82 |
| IFNγ PE XMG1.2 | eBioscience | 12-7311-82 |
| IFNγ PE-Cy7 XMG1.2 | eBioscience | 25-7311-41 |
| IFNγ APC XMG1.2 | eBioscience | 17-7311-82 |
| IFNγ PerCP-Cy5 XMG1.2 | BD | 560660 |
| Ki67 PE SolA15 | eBioscience | 12-5698-82 |
| NK1.1 APC PK136 | BioLegend | 108710 |
| NK1.1 APC-Cy7 PK136 | BioLegend | 108724 |
| P70 S6K Total 49D7 | Cell Signaling | 2708S |
| P70 S6K T389 1A5 | Cell Signaling | 9206S |
| P70 S6K T421/S424 9204 | Cell Signaling | 9204S |
| RORγt PE AFKJS-9 | eBioscience | 12-6988-82 |
| RORγt APC AFKJS-9 | eBioscience | 17-6988-82 |
| S6 S235/236 Pacific Blue D57.2.2E | Cell Signaling | 8520S |
| Streptavidin PE-Cy7 | BD | 405206 |
| Tbet PE eBio4B110 | eBioscience | 12-5825-82 |
| Tbet APC eBio4B110 | eBioscience | 17-5825-82 |
| Thy1.1 APC 53–2.1 | BD | 561974 |
| Thy1.1 PerCP-Cy5 HIS51 | eBioscience | 45-0900-82 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| IL-1β | R&D | 401-ML-025 |
| IL-12 | R&D | 419-ML-050 |
| IL-23 | R&D | 1887-ML-010 |
| IL-4 | R&D | 404-ML-10 |
| IL-6 | R&D | 406-ML-005 |
| Tgfβ | R&D | 240-B-10 |
| Igf | Gropep | CU020 |
| Igf2 | Gropep | FU020 |
| desIgf1 | Gropep | DU100 |
| desIgf2 | Gropep | MU020 |
| Golgiplug | BD | |
| Phorbol 12-myristate 13-acetate | Sigma-Aldrich | P185–1MG |
| Ionomycin | EMD Bioscience | 4D7–952 |
| Critical Commercial Assays | ||
| CD4+ T Cell Isolation Kit | Miltenyi | 130-104-454 |
| LS Columns | Miltenyi | 130-042-401 |
| Dyna Beads | Invitrogen | 11445D |
| SsoAdvanced Universal SYBR Green Supermix | Bio-Rad | 1725275 |
| iScript cDNA Synthesis Kit | Bio-Rad | 1708891 |
| MOG35-55/CFA Emulsion PTX | Hooke Labs | EK-2160 |
| Mitochondrial Stress Test | Agilent | 105015–100 |
| Glycolytic Rate Assay | Agilent | 103344–100 |
| Qiazol | Qiagen | 217084 |
| miRNeasy | Qiagen | 79306 |
| TruSeq Stranded mRNA Library Prep Kit | Illumina | RS-122-2103 |
| Deposited Data | ||
| RNA-Seq, CD4 T Cells | GEO Database | GSE114733 |
| RNA-Seq, Innate Lymphooid Cells | GEO Database | GSE37448 |
| Experimental Models: Cell Lines | ||
| Mouse primary T lymphocytes | Generated in house | |
| Experimental Models: Organisms/Strains | ||
| Igf1rtm1Jcbr | Kloting et al., 2008 | MGI:3818453 |
| Igf1rtm2Arge/J | Jackson Laboratory | Stock No: 012251 |
| Foxp3tm2Ayr | Fontenot et al., 2005 | MGI:3574964 |
| B6-LY5.2/Cr | Charles River | Strain Code 564 |
| B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J | Jackson Laboratory | Stock #022071 |
| 10BiT Tg(Il10-Thy1a)1Weav | Generated in house | MGI:3767675 |
| IFNγ.Thy1.1 (Ifngtm1(Thy1)Weav) | Generated in house | MGI:3831296 |
| IL-17a.hCD2 | Generated in house | N/A |
| RorcCre/+ | Provided by Gerard Eberl | MGI:3054098 |
| Oligonucleotides | ||
| B2m Forward GGTCTTTCTGGTGCTTGTCT | Generated in house | |
| B2m Reverse TATGTTCGGCTTCCCATTCTC | Generated in house | |
| Csf2 Forward GAAGATATTCGAGCAGGGTCTAC | Generated in house | |
| Csf2 Reverse CTTGTGTTTCACAGTCCGTTTC | Generated in house | |
| Foxp3 Forward CCCAGAGTTCTTCCACAACAT | Generated in house | |
| Foxp3 Reverse TTGAGTGTCCTCTGCCTCT | Generated in house | |
| Gata3 Forward TCTGGAGGAGGAACGCTAAT | Generated in house | |
| Gata3 Reverse GGTCTGGATGCCTTCTTTCTT | Generated in house | |
| Ifng Forward CTCTTCCTCATGGCTGTTTCT | Generated in house | |
| Ifng Reverse TTCTTCCACATCTATGCCACTT | Generated in house | |
| Igf1r Forward ACCATCGATTCGGTGACTTCTGCT | Generated in house | |
| Igf1r Reverse AAGGACAAGGAGACCAAGGCATGA | Generated in house | |
| Igf2r Forward AACATTTGTGTGCCCATCTGAGCG | Generated in house | |
| Igf2r Reverse ATTGGGCCAAGGGACTAAGGTCAA | Generated in house | |
| Igfbp4 Forward TCGGAAATCGAAGCCATCCAGGAA | Generated in house | |
| Igfbp4 Reverse GGGTTGAAGCTGTTGTTGGGATGT | Generated in house | |
| Il3 Forward AGGACCCTCTCTGAGGAATAAG | Generated in house | |
| Il3 Reverse TGTAGGCAGGCAACAGTTAAG | Generated in house | |
| Il10 Forward TTGAATTCCCTGGGTGAGAAG | Generated in house | |
| Il10 Reverse TCCACTGCCTTGCTCTTATTT | Generated in house | |
| Il17a Forward CAAACATGAGTCCAGGGAGAG | Generated in house | |
| Il17a Reverse GCTGAGCTTTGAGGGATGAT | Generated in house | |
| Il22 Forward CGACCAGAACATCCAGAAGAA | Generated in house | |
| Il22 Reverse GAGACATAAACAGCAGGTCCA | Generated in house | |
| Rorgt Ex2–4 Forward GAAGACCCACACCTCACAAA | Generated in house | |
| Rorgt Ex2–4 Reverse CAGGAGTAGGCCACATTACAC | Generated in house | |
| Rorgt Ex10–11 Forward TGCAAGACTCATCGACAAGG | Generated in house | |
| Rorgt Ex10–11 Reverse TCCTTATAGAGTGGAGGGAAGG | Generated in house | |
| Tbx21 Forward AGTTCAACCAGCACCAGAC | Generated in house | |
| Tbx21 Reverse CACATCCACAAACATCCTGTAATG | Generated in house | |
| Tgfb3 Forward CGCTACATAGGTGGCAAGAA | Generated in house | |
| Tgfb3 Reverse CAAGTTGGACTCTCTCCTCAAC | Generated in house | |
| Recombinant DNA | ||
| Mouse IL-17a BAC | CHORI | RP24–305023 |
| pL451 | Liu et al. 2003 | N/A |
| pL253 | Na et al. 2013 | N/A |
| pBSK | Addgene | 67504 |
| Human CD2 Y91D | Wolff et al. 1990 | N/A |
| Software and Algorithms | ||
| STAR (version 2.5.3) | Dobin et al., 2013 | |
| SAMtools (version 0.1.18) | Li et al., 2009 | |
| HTSeq (version 0.7.2) | Anders et al., 2015 | |
| DESeq2 (version 1.18.1) | Love et al., 2014 | |
| R (version 3.4.3) | Htt[s://www.r-project.org | |
| ASHR | Stephens et al., 2017 | |
| fgsea R package (version 1.4.0) | https://github.com/ctlab/fgsea/ | |
| MSigDB | Liberzon et al., 2011 |