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. 2021 Apr 27;16(4):e0250109. doi: 10.1371/journal.pone.0250109

Fig 1. Gene expression differences between IPF, ADC and normal control lung.

Fig 1

Total RNA was isolated from stromal cells cultured from lung adenocarcinoma (ADC, n = 4) and the corresponding histologically normal control lung (n = 4) as well as from idiopathic pulmonary fibrosis (IPF) patients (n = 4); the specimens were subjected to microarray analysis and quantitative real-time reverse transcriptase polymerase chain reaction analysis (qRT-PCR). (A) Venn diagram showing the number of either up- or down-regulated genes in stromal cells derived from IPF and ADC compared to control cells. (B) Number of differentially expressed genes in different matrisome categories between IPF, ADC and normal control lung. (C) Validation of genes of interest by qRT-PCR analysis. Data were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and represented graphically as the fold change (FC) as compared to control cell lines. Data are presented as means with each dot representing an individual cell line. The mRNA expression of COL4A1 was lower while the mRNA expression of MMP1 and MMP3 was higher in cells derived from IPF compared to cells derived from ADC or control. POSTN expression was higher in both IPF and ADC derived cells compared to control.