Skip to main content
. 2021 Apr 15;17(4):e1009505. doi: 10.1371/journal.ppat.1009505

Fig 5. Type I IFN, MxA and Ig expression in PBMC stimulated with I-TBEV altered by temperature.

Fig 5

PBMC were left untreated (NS) or stimulated with R848 (5 μM), I-TBEV (dilution 1:12.5) and I-TBEV altered by treatment for 10 minutes at 100°C (I-TBEV ALT 100°C) or for 4 weeks at 42°C (I-TBEV ALT 42°C). (A) The production of IFN-α was measured by ELISA in culture supernatants collected after 24 hours. The results shown were mean values ± SEM of 4 independent experiments. ANOVA p value for IFN-α: 0.012. Based on LSD (equivalent to no adjustments). (B) Relative expression of MxA on 24 hours-collected RNA samples was measured by q-PCR analysis. All quantification data are normalized to the GAPDH level by using the equation 2−ΔCt. The results shown were mean relative values ± SEM of 3 independent experiments. ANOVA p value for MxA: 0.000. Based on LSD (equivalent to no adjustments). (C) The levels of total IgM and IgG were measured by ELISA in culture supernatants collected after 10 days of stimulation. The results shown were mean values ± SEM of 3 independent experiments. ANOVA p value for IgM: 0.033; for IgG: 0.003. Based on LSD (equivalent to no adjustments). (D) Viral RNA copy number was measured by digital PCR analysis on I-TBEV preparations before and after temperature treatment. The results shown were mean values ± SEM of 2 independent experiments. n.s.: not significant.