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. 2021 Apr 15;17(4):e1009366. doi: 10.1371/journal.pgen.1009366

Fig 6. FtsIK211I and FtsWE289G rescue inactive FtsL mutants.

Fig 6

Plasmids pDSW208, pSEB429 (pDSW208, P204::ftsW), pLY91 (pDSW208, P204::ftsI) or their derivatives carrying different ftsW or ftsI alleles were transformed into strain SD399 (W3110, ftsL::kan /pSD256) harboring plasmid pSD296-L86F (pBAD33, PBAD::ftsLL86F) or pSD296-E87K (pBAD33, PBAD::ftsLE87K) and transformants selected on LB plates with antibiotics and glucose. The next day, a single colony of each resulting strain was subject to spot tests. Plates were incubated at 30°C for 24 hours or at 42°C overnight and photographed.