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. 2021 Apr 15;17(4):e1009366. doi: 10.1371/journal.pgen.1009366

Fig 9. FtsBE56A and FtsIK211I suppress FtsWA270T but not FtsWM269K.

Fig 9

(A) Spot test to assess the ability of FtsBE56A to suppress FtsWA270T and FtsWM269K. Plasmid pDSW208, pSEB429 or its derivatives carrying different ftsW alleles were transformed into strain SD367 [TB28, ftsW::kan/pSD257(pSC101ts, ftsW)] or SD368 [TB28, ftsBE56A, ftsW::kan/pSD257(pSC101ts, ftsW)] on LB plates with ampicillin, kanamycin and spectinomycin at 30°C. The spot test was performed as in Fig 5. (B) Spot test of the ability of FtsIK211I to suppress FtsWA270T and FtsWM269K. The test was done in strain SD292 [W3110, ftsW::kan recA56 slrD::Tn10 /pSD257 (pSC101ts, ftsW)] containing plasmid pLY91 (P204::ftsI) or pLY105 (P204::ftsIK211I). SD292 was transformed with plasmid pBAD33 carrying different ftsW alleles and transformants selected at 30°C. Test was done as (A) on LB plates with or without arabinose and IPTG at 30°C and 42°C.