Fig 10. ftsI^K211I and ftsW^E289G are intragenic suppressors of inactive FtsI and FtsW mutants respectively.

(A) ftsI^K211I suppresses inactive FtsI mutations. To test the ability of various FtsI mutants to be rescued by K211I, plasmid pDSW208, pLY91 (pDSW208, P₂₀₄::ftsI) or its derivatives carrying different alleles of ftsI were transformed into strain MCI23ΔrecA (W3110, recA::spec, ftsI23) at 30°C. Complementation test was done at 42°C (to inactivae FtsI23) and in the presence of IPTG to induce the ftsI alleles contained on the plasmids. (B) ftsW^E289G suppresses inactive FtsW mutants. To test the ability of E289G to rescue deficient FtsW alleles, plasmid pDSW208, pSEB429 or its derivatives carrying different alleles of ftsW were transformed into strain SD237 (W3110, leu::Tn10, ftsW::kan/pDSW406 [pBAD33, P_BAD::ftsW]) on LB plates with ampicillin, kanamycin, chloramphenicol and 0.2% arabinose at 37°C. The complementation test was done by removing arabinose (to deplete WT FtsW) and adding IPTG to induce the ftsW alleles on the plasmids.