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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: FASEB J. 2021 May;35(5):e21552. doi: 10.1096/fj.202100027R

Figure 1. Indole metabolites inhibit neutrophil myeloperoxidase activity.

Figure 1.

(A) Primary human PMN lysates were incubated with or without indole derivatives at 1 mM, and myeloperoxidase (MPO) activity was measured by ABTS assay at 405nm. (B) Recombinant human MPO (rhMPO) was incubated in the presence various indole metabolites at various doses and measured by ABTS. Data represented as percent of maximum absorbance at 405nm. All concentrations of indole compounds significantly reduced A405 by one-way ANOVA (**** p < 0.0001). (C) Expression of il8 in T84 cells was evaluated by qRT-PCR after treatment with either 10 μg/mL rhMPO or rhMPO + 0.833 mM indole as described in the Materials & Methods section. Gene expression was normalized to actb and compared with untreated control cells. p < 0.001 using t test (two tailed, two sample heteroscedastic).