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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: FASEB J. 2021 May;35(5):e21552. doi: 10.1096/fj.202100027R

Figure 3. Indole interacts with and binds MPO by microscale thermophoresis (MST).

Figure 3.

(A) Indole binds to rhMPO at a higher affinity compared to a commercially available MPO inhibitor, (B) 4-ABAH. MST assays yielded the indicated KD values: Indole: 272 ± 77.4 μM; 4-ABAH: 409 ± 66.7 μM. Sixteen serial dilutions of every ligand were mixed with fluorescently labeled his-tagged rhMPO in standard capillary tubes. (Data are presented as mean ± SEM).