Table 9. The Effect of P85, F68 and P105 on the Apparent Permeability of [³H(G)]pentamidine (9 nM) MDR1-MDCK Cell Monolayers in the Apical to Basolateral Direction and the Basolateral to Apical Direction.

The percentage recovery of pentamidine is also shown. All the data has been corrected for extracellular space by subtracting [¹⁴C(U)]sucrose (5.5 μM) P_app values which ranged from 0.89 to 2.00 x 10⁻⁶ cm/s. Each value represents three replicates for each n and n = 3. n.d. = not determined as integrity of the barrier compromised.

[3H(G)]Pentamidine (9 nM)	Pluronic Concentration (%)	Papp A2B (10−6 cm/s)	Papp B2A (10−6 cm/s)	A2B (%)	B2A (%)
		Mean±SEM	Mean±SEM	Mass balance	Mass balance
	0	0.678±0.025	0.776±0.062	84	85
	0.01% P85	0.310±0.142	0.431±0.161	86	87
	0.1% P85	0.561±0.0.172	0.227±0.081	89	89
	0.5% P85	n.d.	n.d.	90	90
	0.01% P105	0.577±0.0710	0.818±0.086	86	89
	0.1% P105	0.898±0.161	0.776±0.054	89	88
	0.5% P105	n.d.	n.d.	91	91
	0.01% F68	0.200±0.115	0.106±0.061	95	83
	0.1% F68	0.221±0.067	0.033±0.019	98	87
	0.5% F68	0	0	98	84