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. 2021 Apr 15;15(4):e0009276. doi: 10.1371/journal.pntd.0009276

Table 10. Uptake of [3H(G)]pentamidine into brain tissue (corrected for vascular/[14C]sucrose space) and CSF (corrected for blood/[14C]sucrose contamination)at 2 hours post-injection in BALB/c mice.

Data is presented (a) as the tissue/plasma ratio and converted into concentrations in ng/g of tissue (b) and as concentration for the terminal plasma and CSF samples (c). A limitation of measuring pentamidine by scintillation counting is that any metabolites produced during the 2 hours that have retained the radiolabel, will be counted as [3H(G)]pentamidine. These metabolites may have different transport characteristics and may or may not be active against trypanosomes.

(a) RTISSUE/PLASMA% (mean±SEM)
Region Control (15.7 nM pentamidine) (n = 6) 0.025% F68 + (15.7 nM pentamidine) (n = 6) 0.5% F68 + (15.7 nM pentamidine) (n = 5)
Right brain 115.52 (± 12.46) 120.29 (± 17.14) 87.36 (± 20.36)
Left brain 152.29 (± 33.48) 111.85 (± 19.15) 106.10 (± 12.92)
Cerebellum 204.02 (± 35.28) 208.87 (± 28.81) 172.48 (± 30.34)
Midbrain 181.18 (± 45.30) 254.02 (± 35.48) 180.00 (± 32.83)
Homogenate 249.41 (± 35.59) 184.18 (± 35.22) 293.81 (± 122.95)
Supernatant 123.35 (± 28.45) 99.72 (± 9.02) 98.66 (± 9.47)
Pellet 479.72 (± 72.50) 310.63 (± 38.62) 536.52 (± 212.72)
Choroid plexus 24666.66 (± 4928) 19628.89 (± 4672) 20463.70 (± 1827)
Pituitary gland 15053.41 (± 3598) 11285.42 (± 2008) 15061.87 (± 5321)
(b) Mean concentration (ng/g or ng/ml for the supernatant ±SEM)
Region Control (15.7 nM pentamidine) (n = 6) 0.025% F68 (15.7 nM pentamidine) (n = 6) 0.5% F68 + (15.7 nM pentamidine) (n = 5)
Right brain 0.363 (± 0.035) 0.417 (± 0.061) 0.302 (± 0.058)
Left brain 0.472 (± 0.084) 0.383 (± 0.063) 0.375 (± 0.048)
Cerebellum 0.607 (± 0.032) 0.719 (± 0.097) 0.591 (± 0.084)
Midbrain 0.494 (± 0.075) 0.866 (± 0.115) 0.614 (± 0.072)
Homogenate 0.820 (± 0.183) 0.643 (± 0.132) 0.988 (± 0.375)
Supernatant 0.363 (± 0.037) 0.345 (± 0.035) 0.351 (± 0.043)
Pellet 1.482 (± 0.151) 1.067 (± 0.125) 1.827 (± 0.662)
Choroid plexus 74.68 (± 11.48) 84.04 (± 5.78) 72.20 (± 7.60)
Pituitary gland 43.76 (± 3.82) 37.58 (± 6.54) 68.13 (± 15.05)
Mean concentration
(c) Control 0.025% F68 0.5% F68
CSF pg/ml (± SEM) 2.669 (± 0.765) 1.948 (± 0.826) 3.592 (± 1.932)
Plasma ng/ml (± SEM) 0.343 (± 0.061) 0.345 (± 0.013) 0.356 (± 0.026)
HHS Vulnerability Disclosure