Table 10. Uptake of [3H(G)]pentamidine into brain tissue (corrected for vascular/[14C]sucrose space) and CSF (corrected for blood/[14C]sucrose contamination)at 2 hours post-injection in BALB/c mice.
(a) | RTISSUE/PLASMA% (mean±SEM) | ||
Region | Control (15.7 nM pentamidine) (n = 6) | 0.025% F68 + (15.7 nM pentamidine) (n = 6) | 0.5% F68 + (15.7 nM pentamidine) (n = 5) |
Right brain | 115.52 (± 12.46) | 120.29 (± 17.14) | 87.36 (± 20.36) |
Left brain | 152.29 (± 33.48) | 111.85 (± 19.15) | 106.10 (± 12.92) |
Cerebellum | 204.02 (± 35.28) | 208.87 (± 28.81) | 172.48 (± 30.34) |
Midbrain | 181.18 (± 45.30) | 254.02 (± 35.48) | 180.00 (± 32.83) |
Homogenate | 249.41 (± 35.59) | 184.18 (± 35.22) | 293.81 (± 122.95) |
Supernatant | 123.35 (± 28.45) | 99.72 (± 9.02) | 98.66 (± 9.47) |
Pellet | 479.72 (± 72.50) | 310.63 (± 38.62) | 536.52 (± 212.72) |
Choroid plexus | 24666.66 (± 4928) | 19628.89 (± 4672) | 20463.70 (± 1827) |
Pituitary gland | 15053.41 (± 3598) | 11285.42 (± 2008) | 15061.87 (± 5321) |
(b) | Mean concentration (ng/g or ng/ml for the supernatant ±SEM) | ||
Region | Control (15.7 nM pentamidine) (n = 6) | 0.025% F68 (15.7 nM pentamidine) (n = 6) | 0.5% F68 + (15.7 nM pentamidine) (n = 5) |
Right brain | 0.363 (± 0.035) | 0.417 (± 0.061) | 0.302 (± 0.058) |
Left brain | 0.472 (± 0.084) | 0.383 (± 0.063) | 0.375 (± 0.048) |
Cerebellum | 0.607 (± 0.032) | 0.719 (± 0.097) | 0.591 (± 0.084) |
Midbrain | 0.494 (± 0.075) | 0.866 (± 0.115) | 0.614 (± 0.072) |
Homogenate | 0.820 (± 0.183) | 0.643 (± 0.132) | 0.988 (± 0.375) |
Supernatant | 0.363 (± 0.037) | 0.345 (± 0.035) | 0.351 (± 0.043) |
Pellet | 1.482 (± 0.151) | 1.067 (± 0.125) | 1.827 (± 0.662) |
Choroid plexus | 74.68 (± 11.48) | 84.04 (± 5.78) | 72.20 (± 7.60) |
Pituitary gland | 43.76 (± 3.82) | 37.58 (± 6.54) | 68.13 (± 15.05) |
Mean concentration | |||
(c) | Control | 0.025% F68 | 0.5% F68 |
CSF pg/ml (± SEM) | 2.669 (± 0.765) | 1.948 (± 0.826) | 3.592 (± 1.932) |
Plasma ng/ml (± SEM) | 0.343 (± 0.061) | 0.345 (± 0.013) | 0.356 (± 0.026) |