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. 2021 Mar 1;2(2):100028. doi: 10.1016/j.xhgg.2021.100028

Figure 4.

Figure 4

Spironolactone inhibits and reverses SGK1 non-canonical activation and morphological changes in HUVECS expressing Cx37-Gly41Cys

(A) SGK1 activation via phosphorylation at serine 78 is seen in cells overexpressing Cx37-Gly41Cys in the absence of spironolactone but is not present with addition of spironolactone at the time of induction of Cx37-Gly41Cys expression or 10 h after. Lysates were prepared at 18 h.

(B and C) Cytoplasmic vacuoles are seen in Cx37-Gly41Cys HUVECs that are not treated with spironolactone (B), but addition of spironolactone reverses this phenotype by 14 h after treatment (C). Images were taken at 24 h after induction of Cx37-Gly41Cys expression. Scale bars, 150 μm.