Figure 5.

Effect of vehicle and Flu-AM4 after A, systemic administration and B, local application in formalin-induced paw licking. In Panel A, 1 h before formalin injection, mice were treated with vehicle (white column) and Flu-AM4 (10 mg/kg ip; black columns). In Panel B, mice were treated with vehicle + formalin in paw (white column) and Flu-AM4 (100 µg) + formalin in paw (black columns). The cumulative licking time were counted for 15 min (phase I) and 50 min (phase II). Data are shown as mean ± SEM of 6 animals per group. ** p < 0.01 and *** p < 0.001 vs. vehicle, unpaired t-test.