Figure 6. Yeast dilution affects Src-induced Tor signaling, tissue growth, and organismal lethality.

(A-B) Dietary restriction of yeast suppresses both Src42A constitutively active (CA)-induced proliferation and phosphorylation of 4EBP. (C) Quantification of the total volume of GFP+ cells (µm³) in A-B. Mann-Whitney test. (D) Quantification of phosphorylated 4EBP in A-B. Two-tailed unpaired t-test. (E) Dietary restriction of yeast reduces organismal lethality caused by Src42A CA expression in the wing disc in a dose-dependent manner. One-way ANOVA with Sidak’s post-test.

Figure 6—figure supplement 1. Yeast dilution does not affect Src-induced p38 phosphorylation.

(A) Yeast dilution does not suppress phosphorylation of p38 induced by Src42A constitutively active (CA). (B) Quantification of phosphorylated p38 in A. Both cell autonomous and non-cell autonomous p38 activation by Src42A CA are not suppressed by dietary restriction of yeast. Two-tailed unpaired t-test. Scale bars, 50 µm.