Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene Drosophila melanogaster | ras | Flybase | FLYB: FBgn0003205 | NA |
| Gene Drosophila melanogaster | src42A | Flybase | FLYB: FBgn0264959 | NA |
| Gene Drosophila melanogaster | vg | Flybase | FLYB: FBgn0003975 | NA |
| Gene Drosophila melanogaster | slpr | Flybase | FLYB: FBgn0030018 | NA |
| Gene Drosophila melanogaster | rpr | Flybase | FLYB: FBgn0011706 | NA |
| Gene Drosophila melanogaster | grim | Flybase | FLYB: FBgn0015946 | NA |
| Gene Drosophila melanogaster | hid | Flybase | FLYB: FBgn0003997 | NA |
| Gene Drosophila melanogaster | bsk | Flybase | FLYB: FBgn0000229 | NA |
| Gene Drosophila melanogaster | lic | Flybase | FLYB: FBgn0261524 | NA |
| Gene Drosophila melanogaster | tor | Flybase | FLYB: FBgn0021796 | NA |
| Gene Drosophila melanogaster | rl | Flybase | FLYB: FBgn0003256 | NA |
| Gene Drosophila melanogaster | p38b | Flybase | FLYB: FBgn0024846 | NA |
| Gene Drosophila melanogaster | mef2 | Flybase | FLYB: FBgn0003256 | NA |
| Gene Drosophila melanogaster | atf2 | Flybase | FLYB: FBgn0265193 | NA |
| Gene Drosophila melanogaster | SamS | Flybase | FLYB: FBgn0005278 | NA |
| Gene Drosophila melanogaster | samtor | Flybase | FLYB: FBgn0035035 | NA |
| Gene Drosophila melanogaster | CG13248 | Flybase | FLYB: FBgn0036984 | NA |
| Gene Drosophila melanogaster | tadr | Flybase | FLYB: FBgn0036984 | NA |
| Gene Drosophila melanogaster | CG9413 | Flybase | FLYB: FBgn0030574 | NA |
| Gene Drosophila melanogaster | jhl-21 | Flybase | FLYB: FBgn0028425 | NA |
| Gene Drosophila melanogaster | sbm | Flybase | FLYB: FBgn0030574 | NA |
| Gene Drosophila melanogaster | CG8757 | Flybase | FLYB: FBgn0036380 | NA |
| Gene Drosophila melanogaster | CG16700 | Flybase | FLYB: FBgn0030816 | NA |
| Gene Drosophila melanogaster | path | Flybase | FLYB: FBgn0036007 | NA |
| Gene Drosophila melanogaster | nprl3 | Flybase | FLYB: FBgn0036397 | NA |
| Gene Drosophila melanogaster | nprl2 | Flybase | FLYB: FBgn0030800 | NA |
| Gene Drosophila melanogaster | iml1 | Flybase | FLYB: FBgn0035227 | NA |
| Gene Drosophila melanogaster | wdr24 | Flybase | FLYB: FBgn0027518 | NA |
| Gene Drosophila melanogaster | wdr59 | Flybase | FLYB: FBgn0032339 | NA |
| Gene Drosophila melanogaster | nup44A | Flybase | FLYB: FBgn0033247 | NA |
| Gene Drosophila melanogaster | mio | Flybase | FLYB: FBgn0031399 | NA |
| Gene Drosophila melanogaster | RpL32 | Flybase | FLYB: FBgn0002626 | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-RasV12 | Iswar Hariharan lab | UAS-RasV12 | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-Src42A CA | Bloomington Drosophila Stock Center | BDSC: 6410 RRID:BDSC_6410 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-Src42A | Tian Xu lab | UAS-Src42A | NA |
| Genetic reagent (Drosophila melanogaster) | vg-Gal4 | Bloomington Drosophila Stock Center | BDSC: 6819 RRID:BDSC_6819 |
NA |
| Genetic reagent (Drosophila melanogaster) | gmr-Gal4 | Iswar Hariharan lab | gmr-Gal4 | NA |
| Genetic reagent (Drosophila melanogaster) | TRE-RFP | Bloomington Drosophila Stock Center | BDSC: 59011 | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-slpr RNAi | Bloomington Drosophila Stock Center | BDSC: 32948 RRID:BDSC_32948 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-slpr RNAi | Vienna Drosophila Resource Center | VDRC ID: 33516 RRID:FlyBase_FBst0460140 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-GFP | Iswar Hariharan lab | UAS-GFP | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-his2B RFP | Iswar Hariharan lab | UAS-his2B RFP | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-miRNA RGH | Siegrist et al., 2010 | PMID:20346676 | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-JNK DN | Iswar Hariharan lab | UAS-JNK DN | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-p38 DN | Bloomington Drosophila Stock Center | BDSC: 59005 RRID:BDSC_59005 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-lic RNAi | Bloomington Drosophila Stock Center | BDSC: 31643 RRID:BDSC_31643 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-Tor RNAi | Bloomington Drosophila Stock Center | BDSC: 34639 RRID:BDSC_34639 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-erk RNAi 1 | Vienna Drosophila Resource Center | VDRC ID: 35641 RRID:FlyBase_FBst0461260 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-erk RNAi 2 | Vienna Drosophila Resource Center | VDRC ID: 109573 RRID:FlyBase_FBst0481239 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-mef2 RNAi | Vienna Drosophila Resource Center | VDRC ID: 15550 RRID:FlyBase_FBst0451917 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-atf2 RNAi | Bloomington Drosophila Stock Center | BDSC: 60124 RRID:BDSC_60124 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-SamS RNAi 1 | Vienna Drosophila Resource Center | VDRC ID: 7167 RRID:FlyBase_FBst0470579 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-SamS RNAi 2 | Vienna Drosophila Resource Center | VDRC ID: 103143 RRID:FlyBase_FBst0475005 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-samtor RNAi | Bloomington Drosophila Stock Center | BDSC: 54010 RRID:BDSC_54010 |
NA |
| Genetic reagent (Drosophila melanogaster) | UAS-eiger | Iswar Hariharan lab | UAS-eiger | NA |
| Genetic reagent (Drosophila melanogaster) | UAS-rpr mts | Herman Steller lab | PMID:20837774 | NA |
| Genetic reagent (Drosophila melanogaster) | w¹¹¹⁸ | Erina Kuranaga lab | w¹¹¹⁸ | NA |
| Genetic reagent (Drosophila melanogaster) | Oregon R | Bloomington Drosophila Stock Center | BDSC: 4269 RRID:BDSC_4269 |
NA |
| Antibody | Rabbit polyclonal phospho H3 antibody | Merck | Cat# 06–570 RRID:AB_310177 |
Immunostaining (1:200) |
| Antibody | Rabbit polyclonal cleaved Drosophila Dcp-1 antibody | Cell Signaling | Cat# 9578 RRID:AB_2721060 |
Immunostaining (1:100) |
| Antibody | Rabbit monoclonal phospho-p38 MAPK antibody | Cell Signaling | Cat# 4631 RRID:AB_331765 |
Immunostaining (1:100) |
| Antibody | Mouse monoclonal phospho-Erk MAPK antibody | Merck | Cat# M8159 RRID:AB_477245 |
Immunostaining (1:100) |
| Antibody | Rabbit monoclonal phospho-4EBP1 antibody | Cell Signaling | Cat# 2855 RRID:AB_560835 |
Immunostaining (1:100) |
| Antibody | Rabbit polyclonal phospho-S6 antibody | Kim et al., 2017 | PMID:28829944 | Immunostaining (1:300) |
| Antibody | Alexa mouse Fluor 568 secondary antibody | Thermo Fisher | Cat# A-11004 RRID:AB_253407 |
Immunostaining (1:300) |
| Antibody | Alexa rabbit Fluor 568 secondary antibody | Thermo Fisher | A-11036 RRID:AB_10563566 |
Immunostaining (1:300) |
| Sequence-based reagent | slpr (primer) | This paper | NA | F: 5'-CTACAAGGGCTTCGATCCGTTG-3 R: 5'-GTTTGCCAGCAGCTCTTCATCAG-3 |
| Sequence-based reagent | slpr (primer) | This paper | NA | F: 5'-CAATCATCTGCAGCAGAAGACGC-3' R: 5'-CATCGGAGAATTTGGAATAGGTGC-3' |
| Sequence-based reagent | SamS (primer) | Obata and Miura, 2015 | PMID:32938923 | F: 5'-GCCAACGGCGTTCATATC-3' R: 5'-GGCATATCCAAACATGATACCC-3' |
| Sequence-based reagent | CG13248 (primer) | FlyPrimerBank | PP18106 | F: 5'-AAACCGATGCCTCAACACCTT-3' R: 5'-CAGTCAGCACGTAGATGCCA-3' |
| Sequence-based reagent | tadr (primer) | FlyPrimerBank | PP20579 | F: 5'-CAGCCCGCTGTAAAACTAGC-3' R: 5'-GGCCAGAGCATCTAGCCAG-3' |
| Sequence-based reagent | CG9413 (primer) | FlyPrimerBank | PP29104 | F: 5'-TGGGGTGGCTTTAATTGTTGG-3' R: 5'-CAGTGCGAACCAGTAAACCG-3' |
| Sequence-based reagent | jhl-21 (primer) | Newton et al., 2020 | PMID:32938923 | F: 5'-TCAAGCGGAAGCTAACACTCA-3' R: 5'-TTCGGTGTAAATAAAGACTCCCG-3' |
| Sequence-based reagent | sbm (primer) | FlyPrimerBank | PP3597 | F: 5'-AATGTGCCAACAAAAACAACGA-3' R: 5'-GTCCCTGATGAGTCGGTCTC-3' |
| Sequence-based reagent | CG8757 (primer) | Newton et al., 2020 | PMID:32938923 | F: 5'-AGAAACGATTGGATCGGGCA-3' R: 5'-ATCTGCCATCTTTTGGACCGA-3' |
| Sequence-based reagent | CG16700 (primer) | FlyPrimerBank | PP25676 | F: 5'-CCTACAAGCTATCTGGAGACCA-3' R: 5'-GAGACCTCCGTTCTTGAAGGC-3' |
| Sequence-based reagent | path (primer) | Newton et al., 2020 | PMID:32938923 | F: 5'-TGTTTGATTTGCGCGGCATT-3' R: 5'-TTCGACCCGCTGTCCACTAT-3' |
| Sequence-based reagent | nprl3 (primer) | FlyPrimerBank | PP28256 | F: 5'-GTTAAACCACAGCTATGCAACCA-3' R: 5'-CAGAGTGGGATGACTGACAAAG-3' |
| Sequence-based reagent | nprl2 (primer) | FlyPrimerBank | PP27923 | F: 5'-TTCAACGCTGCATTCTCACC-3' R: 5'-ATTCCGTGCGTACTTCTGCTG-3' |
| Sequence-based reagent | iml1 (primer) | FlyPrimerBank | PP8389 | F: 5'-CGTGGCTGCAACAAATCCTAC-3' R: 5'-GCCCGATTCTATGCTTATCACA-3' |
| Sequence-based reagent | wdr24 (primer) | FlyPrimerBank | PP3395 | F: 5'-GCCCTGGCCCTGAATAAGG-3' R: 5'-TGAAGCCATTGCTGTTTATGGAG-3' |
| Sequence-based reagent | wdr59 (primer) | FlyPrimerBank | PP2977 | F: 5'-GCACCCGAACAAACGTACATC-3' R: 5'-CCGAGTAATCAACCGACATGG-3' |
| Sequence-based reagent | nup44A (primer) | FlyPrimerBank | PP28620 | F: 5'-GAGGAGGTGATTGGCGAAAAG-3'' R: 5'-GCGAGTCTACAAGGGTGGTG-3' |
| Sequence-based reagent | mio (primer) | NA | PMID:26024590 | F: 5'-AGCGAGACGAGCTAAACAATTC-3' R: 5'-GTGTAAGAGGCAAGCAAAGGTT-3' |
| Sequence-based reagent | RpL32 (primer) | This paper | NA | F: 5'-CCAGCATACAGGCCCAAGATCGTG-3' R: 5'-TCTTGAATCCGGTGGGCAGCATG-3' |
| Commercial assay or kit | Methionine analog homopropargylglycine (HPG)- based on Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay kit Single Cell 3’ Library and Gel Bead Kit v2 | Invitrogen | C10428 | NA |
| Commercial assay or kit | Maxwell RSC simply RNA Tissue Kit | Promega | AS1340 | NA |
| Commercial assay or kit | ReverTra Ace qPCR RT Kit | Toyobo | FSQ-101 | NA |
| Commercial assay or kit | BCA protein assay kit | Thermo Fisher | 23225 | NA |
| Software, algorithm | ImageJ | NA | https://imagej.nih.gov/ij/ RRID:SCR_003070 | NA |
| Software, algorithm | IMARIS 9.5.1 | Oxford Instrument |
https://imaris.oxinst.com/packages
RRID:SCR_007370 |
NA |
| Other | DAPI | Sigma | D9542 | 1:1000 |