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. 2021 Mar 24;10:e63532. doi: 10.7554/eLife.63532

Figure 4. Engulfment of AFD–NRE by AMsh glia requires the phosphatidylserine receptor PSR-1 and integrin PAT-2.

(A) Schematic of the genetic pathway underlying apoptotic corpse engulfment in C. elegans. (B–D) Population counts of animals with AMsh glia puncta. Refer Figure 2C for data presentation details. (+) p<0.05 compared to wild type, (–) p≥0.05 compared to wild type. (B) Alleles used in this graph: tat-1(tm3110), tat-1(tm1034), scrm-1(tm805), and ced-8(n1819). (C) Alleles used in this graph: ced-1(e1754), ced-1(e1735), ced-7(n2094), and ced-6(n1813). (D) Alleles used in this graph: psr-1(tm469), tat-1(tm1034), and ttr-52(tm2078). (E) Quantification of puncta within AMsh cell soma in listed mutants. Refer Figure 2D for data presentation details. Median puncta counts and N (number of animals): wild type (14 ± 1 puncta, n = 78 animals), psr-1(tm469) (7.4 ± 0.8 puncta, n = 28 animals), and tat-1 (41.6 ± 4.6 puncta, n = 19 animals). (F) Fluorescence micrograph of a transgenic animal with GFP tagged PSR-1 expressed specifically in AMsh glia (magenta) localizing on the apical membrane around AFD–NRE (green). GFP:PSR localizes to apical membrane in AMsh glia (yellow arrow) around AFD–NRE (asterisk). Scale bar: 5 μm. (F’) Zoom of box in two-color merged image. (G) RNAi (control pat-2) in wild-type or psr-1(tm469) mutant animals. Refer Figure 2C for data presentation details. EV: empty vector control. NRE: neuron-receptive ending.

Figure 4—source data 1. Engulfment of AFD–NRE by AMsh glia requires the phosphatidylserine receptor PSR-1 and integrin PAT-2.
Raw data for Figure 4B, C, D, E, G and Figure 4—figure supplement 1A–C. NRE: neuron-receptive ending.

Figure 4.

Figure 4—figure supplement 1. Engulfment of AFD–NRE by AMsh glia does not depend on some RTK or CED-1/MEGF10/Draper.

Figure 4—figure supplement 1.

(A) Percent animals with AFD–NRE-labeled puncta in AMsh glia. X-axis: genotype; Y-axis: percent animals. N: number of animals examined. Alleles as noted. (B, C) Population counts of animals with AMsh glial puncta in animals as noted in the genotype. Refer Figure 2C for data presentation details. Alleles used in either graph: psr-1(tm469), ced-1(e1754), and ttr-52(tm2078). EV: empty vector control. (+) p<0.05 compared to wild type, (–) p≥0.05 compared to wild type. NRE: neuron-receptive ending.