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. 2021 Mar 24;10:e63532. doi: 10.7554/eLife.63532

Figure 7. AMsh glial engulfment of AFD–NRE modulates AFD–NRE shape and animal thermosensory behavior.

(A) AFD–NRE microvilli labeled with GFP in day 3 adult animals of genotypes as indicated. Three representative images are shown for each genotype. Scale bar: 5 μm (B) Quantification of percent animals with defective AFD–NRE microvilli shape. N: number of animals scored. (C–F) Thermotaxis behavior assays for animals of indicated genotype raised at 15°C (C, E) or 25°C (D, F). Animals assayed 24 hr post-mid-L4 larval stage. N: number of animals. NRE: neuron-receptive ending.

Figure 7—source data 1. AMsh glial engulfment of AFD–NRE modulates AFD–NRE shape and animal thermosensory behavior.
Raw data for Figure 7B–F and Figure 7—figure supplement 1B– D. NRE: neuron-receptive ending.

Figure 7.

Figure 7—figure supplement 1. AMsh glial CED-10 tracks neuron activity to regulate AFD–NRE engulfment.

Figure 7—figure supplement 1.

(A) Day 1 AFD–NRE defects in animals expressing constitutive active CED-10G12V in AMsh glia. (B) Proportion of worms with defective AFD–NRE shape on days 1 and 3 of adulthood in animals expressing constitutive active CED-10G12V or dominant negative CED-10T17N. ttx-1 (p767) mutant analysis included for reference. (C, D) Thermotaxis behavior assays for animals of indicated genotype raised at 15°C (C) or 25°C (D). Animals assayed 24 hr post-mid-L4 larval stage. N: number of animals. Genotype as noted. Alleles used for assays: tax-2(p691) and tax-4(p678). NRE: neuron-receptive ending.