Table 2.
α-Amylase inhibitory activities of the crude extract and solvent fractions of H. abyssinica.
| Concentration | Percentage (%) inhibition | ||||
|---|---|---|---|---|---|
| Chloroform fraction | Ethyl acetate fraction | Water fraction | Crude extract | Acarbose | |
| 15.6 μg/ml | 4.58 ± 0.68 | 7.65 ± 0.88 | 10.54 ± 0.99 | 12.64 ± 0.85 | 19.52 ± 0.76 |
| 31.3 μg/ml | 9.36 ± 0.59 | 15.68 ± 0.67 | 18.54 ± 0.67 | 22.51 ± 1.64 | 32.68 ± 0.66 |
| 62.5 μg/ml | 19.65 ± 1.57 | 29.54 ± 0.49 | 30.54 ± 0.57 | 36.25 ± 0.58 | 44.78 ± 1.18 |
| 125.0 μg/ml | 32.58 ± 0.96 | 41.25 ± 1.34 | 45.58 ± 1.28 | 44.65 ± 0.67 | 60.54 ± 0.67 |
| 250.0 μg/ml | 46.95 ± 0.72 | 52.36 ± 0.75 | 59.54 ± 0.87 | 65.67 ± 0.93 | 79.35 ± 0.91 |
| 500.0 μg/ml | 56.87 ± 0.49 | 61.57 ± 0.49 | 68.24 ± 0.67 | 74.52 ± 0.80 | 93.34 ± 0.58 |
| IC50 μg/ml | 21.57 ± 1.03 | 18.73 ± 0.87 | 16.31 ± 0.54 | 14.52 ± 0.94 | 9.82 ± 0.77 |
Note: each value of percent inhibition of α-amylase is presented as means ± SEM (n = 3). Abbreviations: SEM: standard error of mean; IC50: half-maximal inhibitory concentration.