Figure 6.

The YPF formula downregulated the expression levels of MDSCs immunosuppressive-related genes and proliferation-related proteins in vivo. (a) RT-PCR was used to detect the expression levels of STAT3, iNOS, and Arg-1 mRNA in the tumor tissues. (b) Western blot was used to investigate the expression of MDSCs proliferation-related proteins p-STAT3, p-AKT, p-MEK, and p-ERK in the tumor tissues. All data were expressed as the mean ± SD from at least three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.