Figure 2.

Calmodulin and KSR1 interact in cells.A, KSR1+/+ MEFs were lysed in buffer containing 1 mM Ca²⁺ or 1 mM EGTA and 1 mg of cell lysate was immunoprecipitated (IP) with anti-KSR1 antibody. A sample precipitated with mouse IgG was used as the negative control. Lysates not subjected to immunoprecipitation (Input) were processed in parallel. Samples were resolved by western blotting and probed with anti-KSR1 and anti-calmodulin (CaM) antibodies. An empty lane is designated by (#). The data are representative of three independent experiments. B, in total, 1 mg protein lysate from KSR1+/+ MEFs was immunoprecipitated with anti-calmodulin (CaM) antibody in the presence of Ca²⁺ or EGTA. A sample precipitated with mouse IgG was used as the negative control. Samples were processed as described for panel A. An empty lane is designated by (#). The data are representative of three independent experiments. The positions of migration of molecular mass markers are indicated on the left.