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. 2021 Mar 23;296:100577. doi: 10.1016/j.jbc.2021.100577

Figure 4.

Figure 4

Calmodulin influences EGF-induced activation of ERK. KSR1+/+ MEFs (panels A and B), parental MEFs (panels C and D), and KSR1-null MEFs (panels E and F) were cultured in serum-free medium. Vehicle (−) or 40 μM CGS9343B (+) was added for 16 h, followed by the addition of vehicle (−) or 100 ng/ml EGF (+) for 10 min. Cells were lysed, samples were resolved by western blotting, and blots were probed with antibodies to phosphorylated ERK (pERK), total ERK, KSR1, and β-tubulin. The data are representative of at least three independent experiments. B, D, and F, pERK and total ERK bands were quantified with Image Studio 2.0 (LI-COR Biosciences). Graphs depict the ratio of pERK to total ERK in the same sample, with vehicle-treated samples (no CGS9343B or EGF) set as 1.0. Data represent means ± SD of 3 to 7 experiments. ∗p < 0.001; ∗∗p < 0.0001 using one-way ANOVA, with Tukey’s post-hoc test. ns, not significant.