Table 1.
Protein detected | Reference | Dilution for immunoblots |
---|---|---|
Calmodulin | Monoclonal antibody (28) | 1:1000 |
ERK1/2 | Cell Signaling, 9107S | 1:1000 (immunoblots) 1:100 (PLA) |
GST | Santa Cruz, sc-138 | 1:1000 |
KSR1 | Abcam, ab68483 | 1:1000 (immunoblots) |
KSR1 | Santa Cruz, sc-515924 | 3 μg (immunoprecipitation) 1:100 (PLA) 1:100 (immunostaining) |
MEK1 | Cell Signaling, 9122S | 1:1000 |
Myc | Sigma, 06-549 | 1:1000 |
Na+K+ ATPase | Cell Signaling, 3010S Abcam, ab76020 |
1:1000 (immunoblots) 1:100 (immunostaining) |
pERK1/2 | Cell Signaling, 4377S | 1:1000 (immunoblots) 1:100 (PLA) |
Tubulin | Sigma, T5201 | 1:1000 |