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. 2021 Apr 27;4:506. doi: 10.1038/s42003-021-02024-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Hierarchical clustering between primary colon cancers and metastatic colon cancers based on the quantities of non-synonymous SNVs of 23 isoforms in each sample. The color reflects SNV rate by fraction. B Principal component analyses of primary colon cancers and metastatic colon cancers based on the quantities of non-synonymous SNVs of (A). C Pearson’s correlation of primary colon cancers and metastatic colon cancers based on the quantities of non-synonymous SNVs of (A). The color reflects Pearson’s correlation coefficient for the pairing samples. D Pathway analysis of 23 single-nucleotide-variant isoforms showed enrichment in genes involved in HLA/CD74 antigen presentation pathways.