Fig. 2.

Protein expression of PMFBP1 in sperm and HEK293T cells. a Western blotting was performed to test the expression level of PMFBP1 in the normal and patient sperm. b The influence of the c.301A>C mutation on PMFBP1 protein expression levels in HEK293T cells transfected with wild-type and mutant plasmids of PMFBP1 was tested with Western blotting. c The relative expression levels of wild-type and mutant-PMFBP1 (p.T101P) proteins in b represented by bar charts. Information is indicated as the mean and SEM. **P < 0.05. d MG132 was added to the culture medium for 12 h before the cells were collected. The expression levels of wide-type and mutant-PMFBP1 (p.T101P) were measured by western blotting. DMSO treatment alone was used as the control