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Immunofluorescence analysis and acrosomal staining in sperm of patient and control. a The acrosome and nucleus of the sperm are stained with CD46 antibody (red) and DAPI (blue), respectively. b The localization and expression level of PMFBP1 in sperm from the patient and control were demonstrated by immunofluorescence staining. c Representative images of HeLa cells transfected with FLAG-PMFBP1 and mutant plasmids and stained with anti-FLAG antibody. Bar: 10 μm
