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. 2021 Apr 14;8:661956. doi: 10.3389/fmed.2021.661956

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Copyright © 2021 Lin, Chang, Huang, Chung, Liu, Yen and Tseng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative example of multiple nodule dye localization. (a,e) Axial CT imaging with lung window showed two ground-glass nodules in RUL (arrowhead and arrow, respectively). (b,f) An axial CT imaging with lung window during localization showed 22-gauge Chiba needles introduced to the RUL nodules, respectively (arrowhead and arrow). (c,g) Preoperative 3D volume rendering (VR) imaging showed the location of RUL nodules (arrow and arrowhead). (d,h) VATS imaging showed deposition of methylene blue on RUL (arrow and arrowhead). The inflation-deflation intersegmental plane was shown as dash line in h. Wedge resection of a tiny RUL GGN (arrowhead) was done first, followed by RS1b+3ai subsegmentectomy for a larger RUL GGN (arrow) to ensure adequate deep margin. The location of dye marking correlated well with preoperative 3D VR imaging. The pathology result revealed AIS and MIA, respectively.