Fig. 3.
Altered CA1 pyramidal activation in SPW-Rs during stress-state. (A) The relationship between SPW-R duration (20-ms bins) and multiunit activity during SPW-Rs for first (left) and last (right) day of CIS (LMMs: behaviour-state, F(1, 16896) = 578.48, p < 2.22 × 10−16; duration, F(16, 16896) = 474.38, p < 2.22 × 10−16; behaviour-state x duration, F(16, 16896) = 1.955, p = 0.162) and the last day (LMMs: behaviour-state, F(1, 15877) = 1383.89, p < 2.22 × 10−16; duration, F(16, 15877) = 358.20, p < 2.22 × 10−16; behaviour-state x day, F(16, 15877) = 8.954, p<=0.003) of CIS). Green dots on top of graph indicate significant differences between behaviour-states. (B) Within SPW-R average firing rate differs between behaviour-states (two-way ANOVA: behavior-state, F(1, 1074) = 21.262, p = 4.49 × 10−6; day, F(1, 1074) = 1.037, p = 0.309; behaviour-state x day, F(1, 1074) = 8.552, p = 0.003; acute-rest vs acute-stress, p = 5.97 × 10−7; chronic-rest vs chronic-stress, p = 0.736; acute-rest vs chronic-rest, p = 0.019). (C) Percentage of spikes discharged by CA1 pyramidal cells in SPW-R differs between behaviour-states (two-way ANOVA: behaviour-state, F(1, 1074) = 84.83, p < 2.22 × 10−16; day F(1, 1074) = 0.079, p = 0.778; behaviour-state x day, F(1, 1074) = 1.35, p = 0.25; Tukey's HSD: acute-rest vs acute-stress, p = 1.4 × 10−12; chronic-rest vs chronic-stress, p = 3.62 × 10−7). (D) Dependency of pyramidal cell participation in SPW-Rs on their mean firing rate on the first day (acute-rest: slope = 0.848, R2 = 0.55, p < 2.22 × 10−16; acute-stress: slope = 0.96, R2 = 0.40, p < 2.22 × 10−16; likelihood-ratio test (df = 1) = 4.318, p = 0.038) and the last day (chronic-rest: slope = 0.903, R2 = 0.42, p < 2.22 × 10−16; chronic-stress: slope = 0.898, R2 = 0.21, p = 2.97 × 10−13; likelihood-ratio test (df = 1) = 0.006, p = 0.94) of CIS. (E) Pyramidal cell participation in SPW-Rs differs with the progression of stress protocol (two-way ANOVA: behaviour-state, F(1, 1074) = 1.614, p = 0.204; day, F(1, 1074) = 4.951, p = 0.026; behaviour-state x day, F(1, 1074) = 1.967, p = 0.161; Tukey's HSD: acute-rest vs acute-stress, p = 0.235; chronic-rest vs chronic-stress, p = 0.999; acute-rest vs chronic-rest, p = 0.043). (F) Cumulative distribution of pyramidal cell participation in SPW-Rs differs between rest-states on the first day (black) and last day (blue) of CIS (Kolmogorov-Smirnov test: D = 0.142, p = 0.006). All box-plots, represent interquartile range (IQR, 25th-75th percentiles), median is the thick line in the box and whiskers extend to 1.5 times the IQR. *p < 0.05, **p < 0.01, ***p < 0.001. Acute-rest: n = 288 cells, N = 17 mice; acute-stress: n = 282 cells, N = 16 mice; chronic-rest: n = 282 cells, N = 16 mice; chronic stress: n = 226 cells, N = 16 mice). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)