Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 27;11:9052. doi: 10.1038/s41598-021-88690-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SO removal reverses IOP elevation but not glaucomatous neurodegeneration in HF group. (A) Longitudinal IOP measurements of HF SOHU eyes before and after SO removal and CL eyes at different time points after SO injection. (B) Comparisons of average IOP of the SOHU eyes with or without SO-removal at 5wpi. Data are presented as means ± s.e.m, n = 12. ****: p < 0.0001, Student’s t test. (C) Representative OCT images of mouse retina area surrounding ON head at 5wpi. GCC indicated by double end arrows. (D) Quantification of GCC thickness, represented as percentage of GCC thickness in the SO eyes, compared to the CL eyes. Control (CON): n = 13; HF: n = 19; SO-removal: n = 19, ****: p < 0.0001, one-way ANOVA with Tukey’s multiple comparison test. (E) Upper panel, confocal images of wholemount retinas showing surviving RBPMS-positive (red) RGCs of naïve, HF, and SO removal eyes at 5wpi. Scale bar, 100 µm. Middle panel, confocal images of a portion of the peripheral retina showing surviving RBPMS-positive (red) RGCs in the corresponding groups. Scale bar, 20 µm. Lower panel, light microscope images of semi-thin transverse sections of ON stained with PPD from the corresponding groups. Scale bar, 10 µm. (F,G) Quantification of surviving RGCs in the peripheral retina and surviving axons in ON of the corresponding groups at 5wpi, represented as percentage of SO eyes compared to CL eyes. Data are presented as means ± s.e.m, Control (CON): n = 12; HF: n = 22; SO-Removal: n = 22, ***: p < 0.001, ****: p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.