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. 2021 Apr 27;12:2448. doi: 10.1038/s41467-021-22526-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Time traces of ring diameters from mNeonGreen-PBP2B strain (ME7) for untreated (left) and PC19-treated (right) cells with constant PG synthesis model (red) fitted to representative traces (black). Untreated cell trajectories are aligned relative to fitted constriction start time (black dotted line on left panel). PC19-treated cell trajectories are aligned relative to the arrival time of PC19-laced media (black dotted line on right panel). b Constriction time before and after PC19 treatment for both fast growth (rich media 37 °C) and slow growth (poor media, 30 °C) conditions. c Constriction time of mNeonGreen-PBP2B cells in rich and poor media at two growth temperatures. d mNeonGreen-FtsZ (bWM4) treadmilling speeds in different media and temperature. Top panels, b–d: violin plots; white circle, sample median; thick black lines, interquartile range; thin black lines, 1.5x interquartile range. Bottom panels, b–d: Bottom panels: DABEST plots showing effect size analysis, compared to leftmost condition; black circles, median difference; error bars, 95% confidence interval of median difference. Source data for this figure are provided as a source data file.