Figure 3.

Recruitment of NK and T cells to tumor cells via binding of NKG2D-CD3/CD16. The Indicated sarcoma cell lines were cultivated with PBMC of healthy donors (E:T 2.5:1) in the presence or absence of NKG2D-CD3/CD16 (2.5 µg/ml) and subsequently stained for α-Tubulin and (A) CD16 or (B) CD3. DAPI was used for counterstaining. Interaction of CD16⁺ and CD3⁺ cells was quantified by counting triple positive cells located at sarcoma cells. Grey and black dots represent individual analysis per FoV (n=12) out of three independent experiments.