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. 2021 Apr 14;9:649277. doi: 10.3389/fcell.2021.649277

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Copyright © 2021 Wang, Wang, Kong, Shi, Wang, Chai, Xu and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Osteogenic induction and ADM2 treatment gradually promote the pro-angiogenic ability of BMSCs. (A,B) Representative images (A) and quantification of tube formation (B) in endothelial cells stimulated with conditioned medium from BMSCs treated with complete medium, OIM, ADM2, and OIM + ADM2 (1000 nM). Scale bar: 200 μm. (C–F) Endothelial cells motility in each group was evaluated using the Transwell migration assay (C,D) and the scratch wound assay (E,F). Scale bar: 200 μm. The data were confirmed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test from three independently repeated tests and are presented as the means ± SD. *P < 0.05, **P < 0.01.