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. 2021 Apr 14;9:649277. doi: 10.3389/fcell.2021.649277

FIGURE 7.

FIGURE 7

ADM2 improves vascularized bone regeneration within the tibial distraction area. (A) Representative images of H&E, Masson, and Safranin O-Fast Green staining and immunohistochemical analysis of OCN in control and ADM2 groups. Scale bar: 200 μm. (B) Micro-CT observation of the newly formed blood vessels perfused with Microfil in the distraction regions. (C) Quantitative analysis of the vessel volume fractions within the distraction gaps from each group (n = 3). (D) Immunohistochemical analysis of β-catenin in control and ADM2 groups. (E) Quantitative analysis of the immunohistochemical staining of β-catenin (n = 3). (F) Immunofluorescence staining images of CD31 and EMCN for the distraction area sections from each group. Scale bar: 400 μm. (G) Quantitative analysis of CD31hiEMCNhi cells per mm2 from the staining results (n = 3). The data were confirmed by Mann–Whitney U-test between control group and ADM2 group. *P < 0.05, **P < 0.01.