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. 2021 Apr 14;12:651357. doi: 10.3389/fimmu.2021.651357

Figure 2.

Figure 2

Expression of TIGIT is differentially associated with high IL-21-producing populations in Tfh and Th cells. (A) Single cells in Tfh and Th fractions were plotted by tSNE. Heat maps show expression levels of labeled markers (TIGIT and IL-21). Blue arrows indicate that TIGIT-Th cells express high levels of IL-21. Red arrows show that TIGIT +Tfh cells express high levels of IL-21. (B–E) CD4+ T cells were stimulated for 4 hours with phorbol myristoyl acetate and ionomycin. The proportion of intracellular IL-21 production in TIGIT+ or TIGIT-Tfh and TIGIT+Th or TIGIT-Th cells was measured by flow cytometry. (B, D), representative image. (C, E), multiple comparison of the proportion of IL-21-producing cells (n=6). (C, E) paired t-test. ****p<0.0001, **p<0.01.