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. 2021 Apr 17;6(11):3962–3975. doi: 10.1016/j.bioactmat.2021.04.007

Fig. 6.

Fig. 6

Collagen deposition and angiogenesis evaluation of TAP30% hydrogel. (a) Masson staining of regenerated skin wounds for Tegaderm film, PMI hydrogel, TAP30% hydrogel, and control groups on day 14. (b) Quantitative analysis of relative percentage of collagen deposition coverage area for different groups. (c) Immunofluorescence staining of CD31 in regenerated skin tissue for Tegaderm film, PMI hydrogel, TAP30% hydrogel, and control groups on day 14. CD31 was labeled in red fluorescence and the cell nucleus was labeled in blue fluorescence. (d) Quantitative analysis of the relative percentage of CD31 fluorescence coverage area for different groups. (e) Immunohistochemical staining of CD31 in regenerated skin tissue for Tegaderm film, PMI hydrogel, TAP30% hydrogel, and control groups on day 14. Red arrows represent the location of blood vessels. (f) Relative capillary density in different groups. Mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.