Table 1.
Frag. | OGT residual activity [%] | ||
---|---|---|---|
Fluorescence activity assay | UDP-Glo™ assay | ||
F20 | 41 ± 5 | 60 ± 1 | |
3b | 44 ± 5 | 48 ± 2 | |
4b | 82 ± 7 | 94 ± 2 | |
4c | 65 ± 9 | 9 ± 0 | |
Comp. | OGT residual activity [%] | ||
Fluorescence activity assay | UDP-Glo™ assay | ||
6a | 77 ± 5 | 29 ± 1 | |
6b | 21 ± 1 | 68 ± 0 | |
6c | 88 ± 4 | 83 ± 1 | |
6d | 65 ± 1 | 75 ± 0 | |
6e | 72 ± 0 | 95 ± 2 | |
6f | 77 ± 8 | 40 ± 0 | |
7a | 63 ± 3 | 64 ± 1 | |
8a | 64 ± 0 | 85 ± 0 | |
8b | 64 ± 4 | 69 ± 1 | |
8c | 79 ± 0 | 77 ± 1 | |
8d | 71 ± 4 | 100 ± 0 | |
OSMI-4* | 0 ± 0 | 0 ± 0 |
Results are presented as mean ± SD from two independent experiments, each performed in triplicate. Compounds were tested at 240 μM;
OSMI-4 was tested at 50 μM.