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. 2021 Apr 14;12:658961. doi: 10.3389/fpls.2021.658961

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Copyright © 2021 Doner, Seay, Mehling, Sun, Gidda, Schmitt, Braus, Ischebeck, Chapman, Dyer and Mullen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of EARLY RESPONSIVE TO DEHYDRATION 7 (ERD7) in plant cells. Representative confocal laser-scanning microscopy (CLSM) images (z-sections) of either (A) Agrobacterium-infiltrated Nicotiana benthamiana leaf epidermal cells, (B) biolistically-bombarded Arabidopsis suspension-cultured cells, or (C) transgenic Arabidopsis seedling leaf epidermal and hypocotyl cells, transiently- or stably-transformed with ERD7-mCherry, mCherry-ERD7, or mCherry alone (as indicated by the panel labels), and stained with the neutral lipid dyes BODIPY or MDH (false-colored green). Also shown for each set of images is the corresponding merged image. Arrowheads in the top two rows in (A) indicate obvious examples of mCherry-ERD7 and ERD7-mCherry fluorescence patterns surrounding the BODIPY-stained TAG core, indicating that ERD7 localizes to the surface of LDs. Scale bars in (A–C) = 10 μm.