Figure 1.

Screening and identification of human MUC16^ecto specific antibodies. (A) Schematic representation of MUC16 antigen and the c114 retained ectodomain (MUC16^ecto) which serves as the antibody target. (B) Phage display binding analysis by FACS. Only targets that bind to MUC16^ecto (blue) and not the mutant MUC16 (orange) were selected for sequencing and further development. Examples of negative control (top left) and non-specific binding (top right) are shown. (C) Schematic representation of MUC16^ecto BiTE configuration. (D) Purity of CD3ϵ-congugated MUC16^ecto -specific BiTEDs were validated by SDS-PAGE. Expected band size 50-55 KDa. (E) MUC16^ecto-BiTEDs purity and structural integrity evaluated by SE-HPLC. The SE-HPLC analysis demonstrates the correct apparent molecular weight with the majority of the protein in monomeric form.