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. 2021 Apr 14;12:666162. doi: 10.3389/fphar.2021.666162

FIGURE 5.

FIGURE 5

Neutrophil elastase induces ROS production of chondrocytes. (A,B) ROS levels of chondrocytes were measured by flow cytometry. (A) Representative histograms were shown. (B) Cell associated mean relative fluorescence intensities. Data are mean ± SEM; n = 3. ***p < 0.001. (C,D) Chondrocytes were treated with NE and GSH for 48 h and then stained with annexin V-FITC/PI followed by flow cytometry analysis. (C) The fluorescence pattern of annexin V-FITC/PI staining chondrocytes. (D) Percentages of annexin V positive cells for chondrocytes treated with NE and GSH. Data are mean ± SEM; n = 3. ***p < 0.001.