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. 2021 Apr 17;6(11):3947–3961. doi: 10.1016/j.bioactmat.2021.03.042

Fig. 4.

Fig. 4

In-vitro biocompatibility of GELGYM. a) Representative live-dead images of the human corneal epithelial cells (HCEp), corneal fibroblasts (HCF), and corneal endothelial cells (HCEn) along with hybrid neuroblastoma cells (NDC) cultured on GELGYM hydrogels (FD of 175% and 22.5% w/v and 5 min crosslinking) after 6-day incubation. b) Quantification of metabolic activity using AlamarBlue assay for HCEp, HCF, HCEn, and NDC cultured onto GELGYM hydrogels with varying CT from 3 to 10 min, compared to the cells seeded on the TCP after 2, 4 and 6 days of incubation (ns, *, **, ***, and **** represent p > 0.05, p < 0.05, p < 0.01, p < 0.001 and p < 0.0001). c) Representative stacked confocal live-dead images of HCF, 3-dimensional (3D) cultured within GELGYM hydrogels (FD of 175%, 10% w/v and 5 min CT), during 1 month of incubation. (Green [calcein AM]: lived cells; Red [ethidium homodimer-1]: dead cells). Scale bar: 200 μm.