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. 2021 Mar 24;296:100585. doi: 10.1016/j.jbc.2021.100585

Figure 4.

Figure 4

TIEG1 is involved in PDI-induced tolerogenic DC development. A–D, DEC205+ DCs were isolated from the human blood samples and exposed to PDI (100 ng/ml) in the culture for 48 h. A, immune blots show that a complex of PDI/TIEG1 was detected in DC protein extracts. B, integrated density of the immunoblots in panel A. C, immunoblots show that phosphorylated TIEG1 levels are increased in DCs in response to PDI. D, bars show mean ± SEM of TIEG1 levels in the Foxp3 promoter. E, gated FCS plots show tolerogenic DC counts after exposing to PDI in the culture for 3 days. F, bars show mean ± SEM of tolerogenic DC counts. ∗, p < 0.01, compared with group saline (D) or group a (F). Statistical method: ANOVA with the Dunnett’s test. The data represent three independent experiments (each sample was analyzed in triplicate with average of the three readouts as one datum).