Figure 6.

Administration of PDI alleviates experimental asthma by generating TolDCs in the lung. An asthma mouse model was developed with ovalbumin (OVA) as an antigen and mice were treated with PDI by nasal instillation (A). B, representative lung histology images (original magnification: × 200). C, the curves show mouse airway resistance records in response to methacholine challenge. C–F, bars show the serum levels of OVA-specific IgE (sIgE, D), IL-4 (E), and IL-5 (F). G, bars show the TGF-β levels in BALF. H, bars show eosinophil frequency in cellular components of BALF. I and J, bars show frequencies of TolDC (I) and Treg (J) in lung mononuclear cells. K, bars show serum FITC-dextran (Dex) levels. FCS plots of H–J, are presented in Figs. S1–S3. Group labels in panel C–K are the same as those in panel B. The data of bars are presented as mean ± SEM. Each group consists of six mice. ∗, p < 0.01 (ANOVA with Dunnett’s test), compared with group a. #, p < 0.01 (the Student’s t-test), compared with group b. The data represent three independent experiments (each sample was analyzed in triplicate with average of the three readouts as one datum).