REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD3ε Brilliant Violet 421 (clone SK7)	Biolegend	Cat# 344834; RRID:AB_2565675
Anti-human CD8α Alexa Fluor 488 (clone RPA-T8)	Biolegend	Cat# 301021; RRID:AB_2561281
Anti-human CD4 PE (clone RPA-T4)	eBioscience	Cat# 12-0049-42, RRID:AB_1582249
Anti-human CD45 Brilliant Violet 510 (clone HI30)	Biolegend	Cat# 304036; RRID:AB_2561940
Anti-mouse CD16/32 (clone 2.4G2)	Biolegend	Cat# 101301; RRID:AB_312800
Anti-human/primate EGF biotinylated	R&D Systems	Cat# BAF236; RRID:AB_356307
Streptavidin APC	Biolegend	Cat# 405207
Anti-human ErbB1 (ICR62)	Institute of Cancer Research	N/A
Anti-human ErbB2 (ICR12)	Institute of Cancer Research	N/A
Anti-human ErbB3/HER-3 PE (clone 1B4C3)	Biolegend	Cat# 324706; RRID:AB_2099569
Anti-human ErbB4/Her4 (clone H4.77.16 (Ab77))	Novus	Cat# NB120-3104; RRID:AB_789269
Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary Alexa Fluor Plus 488	Thermo Fisher Scientific	Cat# A32723; RRID:AB_2633275
Goat anti-rat IgG (minimal x-reactivity) APC	Biolegend	Cat# 405407; RRID:AB_315018
7-amino actinomycin D	Cayman Chemical Company	Cat# 11397
Anti-mouse TER-119 PerCP-Cyanine5.5 (clone TER-119)	Thermo Fisher Scientific	Cat# 45-5921-82; RRID:AB_925765
Anti-Hypoxyprobe antibody, PAb2627	Hypoxyprobe	Cat# HP PAb2627
Anti-human CD3 (clone F7.2.38)	Dako	Cat# M7254
Rabbit anti-HIF1α monoclonal (clone EP1215Y)	Abcam	Cat# ab51608; RRID:AB_880418
Donkey anti-mouse IgG (H+L) Alexa Fluor Plus 488	Thermo Fisher Scientific	Cat# A32766; RRID:AB_2762823
Donkey anti-rabbit IgG (H+L) Alexa Fluor 568	Thermo Fisher Scientific	Cat# A10042; RRID:AB_2534017
Anti-mouse CD4 FITC (clone RM4-5)	eBioscience	Cat# 11-0042-82; RRID:AB_464896
Anti-mouse CD8α eFluor 450 (clone 53-6.7)	eBioscience	Cat# 48-0081-82; RRID:AB_1272198
Anti-mouse CD3ε PE (clone 145-2C11)	eBioscience	Cat# 12-0031-82; RRID:AB_465496
Bacterial and virus strains
Stbl3 E. coli	Thermo Fisher Scientific	Cat# C737303
Biological samples
Human blood/T cells	Healthy volunteers	Guy’s and St Thomas’ Research Ethics Committee (REC reference 09/H0804/92)
Chemicals, peptides, and recombinant proteins
Ficoll-Paque PLUS	GE Healthcare	Cat# GE17-1440-02
T4 DNA ligase	Thermo Fisher Scientific	Cat# EL0011
RetroNectin recombinant human fibronectin fragment	Takara	Cat# T100B
Dynabeads human T-activator CD3/CD28	Thermo Fisher Scientific	Cat# 11131D
Restriction endonucleases	New England Biolabs	Various, as per this paper
FuGENE HD transfection reagent	Promega	Cat# E2311
Recombinant human IL-4	PeproTech	Cat# 200-04
Proleukin (aldesleukin), human recombinant IL-2	Clinigen Group	N/A
Polybrene	Santa Cruz Biotechnology	Cat# NC9840454
KiCqStart SYBR green qPCR ReadyMix, with ROX	Sigma-Aldrich	Cat# KCQS02
TRIzol reagent	Thermo Fisher Scientific	Cat# 15596026
Cobalt(II) chloride	Sigma-Aldrich	Cat# 60818
XenoLight D-Luciferin - K+ salt bioluminescent substrate	PerkinElmer	Cat# 122799
RediJect coelenterazine h bioluminescent substrate	PerkinElmer	Cat# 760506
Collagenase I from Clostridium Histolyticum	Sigma-Aldrich	Cat# C0130
DNase I	AppliChem	Cat# A3778
Hypoxyprobe 1 Kit Hpi	Hypoxyprobe	Cat# HP1-200Kit
Dako EnVision + system-HRP (DAB)	Agilent Technologies	Cat# K4010
DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride)	Thermo Fisher Scientific	Cat# D1306
Critical commercial assays
TaqMan gene expression assay	Thermo Fisher Scientific	Cat# 4331182
QIAGEN Plasmid Mini, Midi and Maxi Kits	QIAGEN	Cat# 12125, 12145, 12163
QIAGEN DNeasy Blood & Tissue Kit	QIAGEN	Cat# 69506
Pan T Cell Isolation Kit, human	Miltenyi Biotec	Cat# 130-096-535
EXPRESS One-Step Superscript qRT-PCR Kit	Thermo Fisher Scientific	Cat# 11781200
Human IL-2 ELISA Ready-SET-Go! Kit, 2. Generation	eBioscience	Cat# 15590997
Human IFN-Gamma DuoSet ELISA	R&D Systems	Cat# DY285B
UltraView Universal DAB Detection Kit	Roche	Cat# 760-500; RRID:AB_2753116
UltraView Universal Alkaline Phosphatase Red Detection Kit	Roche	Cat# 760-501
Experimental models: cell lines
SKOV3	ATCC	Cat# HTB-77; RRID:CVCL_0532
HN3	Ludwig Institute for Cancer Research, London	RRID:CVCL_8126
HEK293T	ATCC	Cat# CRL-3216; RRID:CVCL_0063
Experimental models: organisms/strains
NOD-scid IL2Rγnull (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ)	Charles River	RRID:BCBC_4142
Oligonucleotides
gBlocks gene fragments	Integrated DNA Technologies	Various, as per this paper
GCCTACCAAGAACAACTGGAC	Integrated DNA Technologies	Fwd binds upstream AgeI
GGCCCTGCCCCCTCGCGCCCCAGCCGCTGGA	Integrated DNA Technologies	Fwd to fuse CD3ζ-ODD
TCCAGCGGCTGGGGCGCGAGGGGGCAGGGCC	Integrated DNA Technologies	Rev to fuse CD3ζ-ODD
GACTAATCCGGATCCTCGAGTGGCTGTTACTGGA ATACTGTAACTGTGCTTTGAGG	Integrated DNA Technologies	Rev to ODD stop XhoI
CCATGGTGAAGCGTGAGAAAAATG	Integrated DNA Technologies	Fwd to amplify reporter
CTCGAGTTACTTGTACAGCTCGTCCATGC	Integrated DNA Technologies	Rev to amplify reporter
GAGAAGGCCGGCGGTGCCCCAGCCGCTGGA	Integrated DNA Technologies	Fwd to fuse Luc-ODD
TCCAGCGGCTGGGGCACCGCCGGCCTTCTC	Integrated DNA Technologies	Rev to fuse Luc-ODD
CCTCAAAGCACAGTTACAGTATTCCAGGGAAGCGG AGCTACTAACTTCAG	Integrated DNA Technologies	Fwd to fuse Luc-ODD-2A
CTGAAGTTAGTAGCTCCGCTTCCCTGGAATACTGTA ACTGTGCTTTGAGG	Integrated DNA Technologies	Rev to fuse Luc-ODD-2A
GCGGGCCTCTTCGCTATTA	Integrated DNA Technologies	Rev clone 9HRE in SFG
ATCCGCCACAACATCGAG	Integrated DNA Technologies	Fwd clone 9HRE in SFG
Recombinant DNA
SFG CBG99Luc-P2A-EGFP	This study	P1
SFG HRE9 CBG99luc-ODD401-603-P2A-GFP	This study	P20; HypoxiLuc reporter
SFG 4αβ-2A-T1E-CD28-CD3z-ODD	This study	P22
SFG HRE9 4αβ-2A-T1E-CD28-CD3z	This study	P26
SFG HRE9 4αβ-2A-T1E-CD28-CD3z-ODD	This study	P23; HypoxiCAR
Software and algorithms
FlowJo v.10 Software	TreeStar Inc.	https://www.flowjo.com/
Prism 6	GraphPad	https://www.graphpad.com/scientific-software/prism/
Snapgene	GSL Biotech	https://www.snapgene.com/
NIS-Elements Imaging Software	Nikon	https://www.microscope.healthcare.nikon.com/products/software
R version 3.5.1	The R Foundation	https://www.r-project.org/
Other
Hypoxia incubator chamber	STEMCELL Technologies	Cat# 27310
Gas cylinders	BOC	Custom (at indicated gas mixes)