REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD34 antibody	BD Biosciences	Clone 581; RRID: AB_10563908
CD90 antibody	BD Biosciences	Clone 5E10; RRID: AB_395970
CD133 antibody	BD Biosciences	Clone W6B3C1; RRID: AB_2744280
Human CD45 antibody	BD Biosciences	Clone HI30; RRID: AB_2033960
Biological samples
SCD CD34+ cells	NHLBI, NIH	This paper (Donor information was shown in Table S2.)
Healthy donor CD34+ cells	NHLBI, NIH	This paper (Donor information was shown in Table S2.)
Chemicals, peptides, and recombinant proteins
Cas9 protein	MacroLab Facility	University of California, Berkeley, CA, USA
Stem cell factor	R&D Systems	255-SC
FMS-related tyrosine kinase 3 ligand	R&D Systems	308-FK
Thrombopoietin	R&D Systems	288-TP
Erythropoietin	AMGEN	EPOGEN ®
Interleukin 3	R&D Systems	203-IL
Dexamethasone	VETone	501012
Estradiol	Pfizer	DEPO®-ESTRADIOL
Bovine serum albumin	Roche	10738328103
Busulfan	PDL BioPharma	BUSULFEX®
Experimental models: Cell lines
SCD B cell line	This paper	Not applicable
Experimental models: Organisms/strains
NBSGW mice	The Jackson Laboratory	026622
Rhesus macaque	NIH Animal Center	Not applicable
Oligonucleotides
SCD mutation-targeting guide RNA	Synthego Corporation	5′-GUA ACG GCA GAC UUC UCC AC-3′
Normal β-globin gene-targeting guide RNA	Synthego Corporation	5′-GUA ACG GCA GAC UUC UCC UC-3′
Rhesus β-globin-targeting guide RNA	Synthego Corporation	5′-GUG ACG GCA UUC UUC UCC UC-3′
Normal β-globin donor DNA	Integrated DNA Technologies, Inc.	5′-TTC ATC CAC GTT CAC CTT GCC CCA CAG GGC AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG ATG CAC CAT GGT GTC TGT TTG AGG TTG CTA GTG AAC ACA G-3′
βs-globin donor DNA	Integrated DNA Technologies, Inc.	5′-TTC ATC CAC GTT CAC CTT GCC CCA CAG GGC AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG ATG CAC CAT GGT GTC TGT TTG AGG TTG CTA GTG AAC ACA G-3′
Rhesus βs-globin donor DNA	Integrated DNA Technologies, Inc.	5′-TTC ATC CAC GTT CAC CTT GCC CCA CAG GGT GGT GAC GGC ATT CTT CTC CAC AGG AGT CAG ATG CAC CAT GGT GTC TGT TTG AGG TTG CTC GTG AAC ACA G-3′
Primers and probes	This paper	Table S3
Software and algorithms
CRISPResso2	Pinello Lab, Massachusetts General Hospital / Harvard Medical School, Boston, MA, USA	https://crispresso.pinellolab.partners.org/
FlowJo v10	BD Biosciences	https://www.flowjo.com/
JMP 14	SAS Institute Inc	https://www.jmp.com/en_us/home.geo.html
Other
StemSpan SFEM media	StemCell Technologies	09650
Iscove’s Modified Dulbecco’s Medium	Corning	10-016-CV
KnockOut Serum Replacement	Thermo Fisher Scientific	10828028
MaxCyte GT System	MaxCyte, Inc.	https://maxcyte.com/gtx/
Hemoglobin electrophoresis	Helena Laboratories	4063
QuantStudio 6 Flex Real-Time PCR System	Thermo Fisher Scientific	4485689
FACSAria II	BD Biosciences	643180
FACSCanto	BD Biosciences	338960