Fig. 2.

Scn1a expression is reduced in 1b deletion mouse model. a–c Immunofluorescent analysis of Na_V1.1 in wildtype mice across cerebellum (a), hippocampus (b), and cortex (c), regions taken from wildtype in panel f. Scale bars a and b = 100 μm, c = 250 μm. d Bar plot showing relative expression of Scn1a using qPCR in 3-month-old mice (mean ± SEM), values normalized to WT cortex. Scn1a expression reduced in 1b^−/− cortex vs WT cortex (**P = 0.0092), 1b^−/− hippocampus vs WT hippocampus (**P = 0.0029), and 1b^−/− cerebellum vs WT cerebellum (****P < 0.0001). e Western blots of P29-32 mouse brain membrane fractions, showing reduction of Na_V1.1 protein in cortex of 1b^+/− (*P = 0.0174) and 1b^−/− (**P = 0.0014) mice, hippocampus of 1b^+/− (*P = 0.0445) and 1b^−/− (**P = 0.0025) mice and cerebellum of 1b^−/− (*P = 0.0142) mice. f Immunofluorescent analysis of sagittal sections of P28 mice revealed a reduction in Na_V1.1 (green) expression in homozygous versus WT mice with no changes in parvalbumin (red) expression. Scale bars = 1 mm