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. 2021 Apr 28;29(5):689–703. doi: 10.1016/j.chom.2021.04.003

Table 1.

SARS-CoV-2 RNA Detection Technologies

Technology Sample Processing Amplification Method Cas Effector Protein Assay Readout Companion Smartphone App SARS-CoV-2 Target Gene Patient Sample Validation
 Reference
No. of + Samples No. of – Samples Sensitivity (%) Specificity (%)
DETECTR Nucleic acid extraction RT-LAMP Cas12 Lateral flow E 36 42 95 100 Broughton et al., 2020b
N 36 42 95 100
CARMEN Nucleic acid extraction PCR Cas13 Fluorescent Orf1ab n.d. n.d. n.a. n.a. Ackerman et al. 2020
CASdetec Chemical treatment RT-RPA Cas12 Fluorescent Orf1ab n.d. n.d. n.a. n.a. Guo et al. 2020
SHERLOCK Nucleic acid extraction RT-RPA Cas13 Fluorescent S 81 453 100 100 Patchsung et al., 2020
Lateral flow S 81 453 97 100
CRISPR-COVID Nucleic acid extraction RT-RPA Cas13 Fluorescent Orf1ab 52 36 100 100 Hou et al. 2020
CRISPR-FDS-v1.0 Nucleic acid extraction RT-RPA or RT-PCR Cas12 Fluorescent N or Orf1ab 19 10 100 71.4 Huang et al. 2020
CRISPR/Cas12a-NER Nucleic acid extraction RT-RPA Cas12 Fluorescent E 16 15 100 100 Wang et al. 2020
STOPCovidv1 Chemical and heat treatment RT-LAMP Cas12 Lateral flow N 62 22 84 100 Joung et al. 2020
STOPCovidv2 Chemical, heat, and magnetic treatment Fluorescent N 200 200 93.1 98.5
iSCAN Nucleic acid extraction RT-LAMP Cas12 Fluorescent E 26 5 50 100 Ali et al. 2020
Fluorescent N 26 5 88 100
Lateral flow N 21 3 81 100
Electric field-mediated SARS-CoV-2 detection Chemical and heat treatment followed by isotachophoresis extraction RT-LAMP Cas12 Fluorescent N 32 32 90.6 100 Ramachandran et al. 2020
E 32 32 90.6 100
SHINE Chemical and heat treatment (HUDSON) RT-RPA Cas13 Fluorescent X Orf1ab 42 40 93.3 100 Arizti-Sanz et al. 2020
Lateral flow Orf1ab 4 3 100 100
Contamination-free, visual Cas12 assay Nucleic acid extraction RT-LAMP Cas12 Fluorescent X Orf1ab 7 3 100 100 Chen et al. 2020
E n.d. n.d. n.a. n.a.
N n.d. n.d. n.a. n.a.
Direct, Cas13a detection Nucleic acid extraction n.a. Cas13 Fluorescent X E & N combined 5 1 100 100 Fozouni et al. 2020
CRISPR-FDS-v2.0 Chemical and heat treatment of saliva RT-RPA Cas12 Fluorescent Orf1ab 44 59 100 100 Ning et al. 2020
Fluorescent X Orf1ab 44 59 97.7 100
Chemical and heat treatment of nasal swab RT-RPA Cas12 Fluorescent Orf1ab 27 76 100 97.7
Fluorescent X Orf1ab 27 76 100 98.7