| Component | Volume (μL) |
|---|---|
| pX459 (100 ng/μL) | 1 |
| sgRNA solution (80 nM) | 2 |
| Fast Digest Buffer (10×) | 2 |
| DTT (10 mM) | 1 |
| Fast Digest BbsI | 1 |
| T4 DNA Ligase | 0.5 |
| ddH2O | 12.5 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.