REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One ShotTM TOP10 Chemically Competent E. coli	Thermo Fisher Scientific	Cat# C404010
Chemicals, peptides, and recombinant proteins
GlutaMAXTM Supplement	Thermo Fisher Scientific	Cat# 35050061
B-27TM Supplement	Thermo Fisher Scientific	Cat# 17504044
Penicillin-streptomycin	Thermo Fisher Scientific	Cat# 15140122
Human EGF	PeproTech	Cat# AF-100-15
Human FGF-2	PeproTech	Cat# 100-18B
Heparin	MP Biomedicals	Cat# 0210193125
Poly-D-lysine	Sigma-Aldrich	Cat# P7280
Laminin	Roche Diagnostics	Cat# 11243217001
T4 DNA Ligase	Thermo Fisher Scientific	Cat# EL0014
FastDigest BbsI	Thermo Fisher Scientific	Cat# ER1011
T4 Polynucleotide Kinase	Thermo Fisher Scientific	Cat# EK0031
StemProTM AccutaseTM	Thermo Fisher Scientific	Cat# A1110501
Puromycin-Dihydrochloride	Thermo Fisher Scientific	Cat# A1113803
Critical commercial assays
LipofectamineTM 3000 Transfection Reagent	Thermo Fisher Scientific	Cat# L30001
GeneJET Plasmid Miniprep Kit	Thermo Fisher Scientific	Cat# K0503
GeneJET Endo-Free Plasmid Maxiprep Kit	Thermo Fisher Scientific	Cat# K0861
DMEM-F12 without glutamine	Thermo Fisher Scientific	Cat# 21331020
NeurobasalTM medium	Thermo Fisher Scientific	Cat# 21103049
Phusion High-Fidelity DNA Polymerase	Thermo Fisher Scientific	Cat# F530S
Opti-MEMTM Reduced Serum Medium	Thermo Fisher Scientific	Cat# 31985062
QIAamp DNA Micro Kit	QIAGEN	Cat# 56304
QuickExtractTM DNA Extraction Solution	Lucigen	Cat# QE09050
Experimental models: Cell lines
Neural precursor cells from adult mouse hippocampus	Laboratory of Gerd Kempermann	Line MI6
Oligonucleotides
Hcar1_sgRNA1_fw: CACCGGTCGTGCTGTCTCA TCGAG	(Pötzsch et al., 2021)	N/A
Hcar1_sgRNA1_rev: AAACCTCGATGAGACAGCA CGACC	(Pötzsch et al., 2021)	N/A
Hcar1_sgRNA2_fw: CACCGTTCCCAGAGGCCATCTGAC	(Pötzsch et al., 2021)	N/A
Hcar1_sgRNA2_rev: AAACGTCAGATGGCCTCTGGGAAC	(Pötzsch et al., 2021)	N/A
Hcar1_genotyping_fw: AGTTTCTCTCGTGGGTGCAG	(Pötzsch et al., 2021)	N/A
Hcar1_genotyping_rev: TGCTTGCCTGGCTCGAATAA	(Pötzsch et al., 2021)	N/A
Atf3_sgRNA_fw: CACCGGCGGTCGCACTGACTTCTG	This paper	N/A
Atf3_sgRNA_rev: AAACCAGAAGTCAGTGCGACCGCC	This paper	N/A
Atf3_genotyping_fw: GTGGGCCAGAACATAAAGGA	This paper	N/A
Atf3_genotyping_rev: CCAGAGCTTTGGATGGTTCT	This paper	N/A
U6_sequencing: GAGGGCCTATTTCCCATGATTCC	(Ran et al., 2013)	N/A
Recombinant DNA
pSpCas9(BB)-2A-Puro (PX459) V2.0	(Ran et al., 2013)	RRID: Addgene_62988
Software and algorithms
Genome browser (e.g., Ensembl)	http://www.ensembl.org/Mus_musculus/Info/Index	RRID:SCR_013367
CRISPR design tool (e.g., E-CRISP)	http://www.e-crisp.org/E-CRISP/	RRID:SCR_019088
Other
Cell culture flasks (T25)	TPP Techno Plastic Products AG	Cat# 90026
Cell culture plates (96-well)	TPP Techno Plastic Products AG	Cat# 92696
Cell culture plates (6-well)	TPP Techno Plastic Products AG	Cat# 92406
Cell culture plates (384-well)	Corning Incorporated	Cat# 353961