Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 28;12:2460. doi: 10.1038/s41467-021-22814-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Antibiotic resistance phenotypes of PA01:p110820 transformants compared to a plasmid-free PA01 control. Plasmid carriage increased resistance to meropenem and piperacillin-tazobactam to at least 32 mg/L, exceeding the EUCAST clinical breakpoints (meropenem, 8 mg/L; piperacillin-tazobactam, 16 mg/L). B Changes in plasmid copy number during infection. Plotted points show the mean plasmid copy number of lung (blue) and gut (beige) isolates from each time point (+/− s.e.m.; n = 10–12 isolates), excluding two genetically divergent gut isolates that are plotted separately. The second wave of lung infection was associated with a reduced plasmid copy number compared to the initial infection (two-tailed t₅₇ = 8.15, P < 0.0001). C Fitness effects of plasmid carriage were assayed by measuring the growth rate of PA01 and PA01:p110820 in antibiotic-free culture medium (n = 11 replicates/strain). Plasmid carriage reduced growth rate (two-tailed t₂₁ = 2.48, P = 0.0215). D Plasmid copy number of lung isolates according to genotype (mean + /− s.e.m.; n = 7–24 isolates per genotype). Source data are provided as a Source Data file.