Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 28;11:9188. doi: 10.1038/s41598-021-88610-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PKR dimerization assay. (a) Principle of the PKR dimerization assay based on split nanoluciferase: PKR constructs C-terminally tagged with nanoluciferase SmBit and LgBit fragments are co-expressed in PKR-KO cells. Upon poly(I:C) transfection, PKR dimerization leads to the assembly of active nanoluciferase. (b) Kinetics of nanoluciferase activity measured after substrate addition, for wt PKR, in mock-transfected cells and in cells transfected with poly(I:C) for 2 h before substrate addition. (c) Graphs show the mean and SEM of nanoluciferase activity induction following poly(I:C) transfection in cells that co-expressed wt or mutant PKR linked to the Sm and Lg Bits of nanoluciferase (homodimerization assay). Luciferase activity was measured 5 min after substrate addition. Significance is shown for multiple one-way ANOVA comparisons with WT PKR and with the negative control 4 K. Graphs were generated with Excel 2011 (Microsoft). Statistical analysis was performed using Prism7 (Graphpad).